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A Critical Component of Meiotic Drive in Neurospora Is Located Near a Chromosome Rearrangement
- Source :
- Genetics. 197:1165-1174
- Publication Year :
- 2014
- Publisher :
- Oxford University Press (OUP), 2014.
-
Abstract
- Neurospora fungi harbor a group of meiotic drive elements known as Spore killers (Sk). Spore killer-2 (Sk-2) and Spore killer-3 (Sk-3) are two Sk elements that map to a region of suppressed recombination. Although this recombination block is limited to crosses between Sk and Sk-sensitive (SkS) strains, its existence has hindered Sk characterization. Here we report the circumvention of this obstacle by combining a classical genetic screen with next-generation sequencing technology and three-point crossing assays. This approach has allowed us to identify a novel locus called rfk-1, mutation of which disrupts spore killing by Sk-2. We have mapped rfk-1 to a 45-kb region near the right border of the Sk-2 element, a location that also harbors an 11-kb insertion (Sk-2INS1) and part of a >220-kb inversion (Sk-2INV1). These are the first two chromosome rearrangements to be formally identified in a Neurospora Sk element, providing evidence that they are at least partially responsible for Sk-based recombination suppression. Additionally, the proximity of these chromosome rearrangements to rfk-1 (a critical component of the spore-killing mechanism) suggests that they have played a key role in the evolution of meiotic drive in Neurospora.
- Subjects :
- Genes, Fungal
Molecular Sequence Data
Locus (genetics)
Chromosomal rearrangement
Investigations
Biology
Neurospora
Meiosis
Genetics
DNA, Fungal
Gene Rearrangement
Base Sequence
fungi
Chromosome Mapping
Sequence Analysis, DNA
Gene rearrangement
Spores, Fungal
biology.organism_classification
Meiotic drive
Genetic Loci
Mutation
Chromosomes, Fungal
Recombination
Genetic screen
Subjects
Details
- ISSN :
- 19432631
- Volume :
- 197
- Database :
- OpenAIRE
- Journal :
- Genetics
- Accession number :
- edsair.doi.dedup.....bf6a2bdd23d53277f738c78e65e735c0