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Immunomodulating effects of Korean mistletoe lectin in vitro and in vivo
- Source :
- International Immunopharmacology. 9:1555-1561
- Publication Year :
- 2009
- Publisher :
- Elsevier BV, 2009.
-
Abstract
- The immunomodulatory effects of Korean mistletoe lectin (KML), one of the major active components in Viscum album L. var. coloratum, were investigated in vitro in immune cell proliferation and natural killer (NK) cell- and macrophage-mediated cytotoxicity, and in vivo in the forced swim test and cold stress. In mitogen-induced lymphocyte proliferation of murine splenocytes, concanavalin A and lipopolysaccharide significantly increased the proliferation of T cell and B cell lymphocytes, respectively. KML exposure increased lymphocyte proliferation in response to mitogen. KML also increased the splenic NK cell and macrophage activities in vitro. Exposure to KML increased production of cytokines such as interleukin-1 and interleukin-6 by macrophages. Two-week treatment with KML (30, 100, 300 and 600 microg/kg) increased the recruitment of lymphocytes, monocytes and macrophages. In the forced swim test, the immobility time was significantly attenuated by treatment with KML (300 and 600 microg/kg). In a cold stress experiment, spleen and thymus weight increased in KML-treated mice, while the weight of adrenal gland was lower than that in vehicle-treated mice. The levels of serum aminotransferases, lactate dehydrogenase and alkaline phosphatase were decreased by KML treatment. KML treatment also induced increases in the percentages of CD4(+) and CD8(+) cells in thymus. Our results suggest that KML enhances the immune system through modulation of lymphocytes, NK cells, and macrophages.
- Subjects :
- Cytotoxicity, Immunologic
Male
medicine.medical_specialty
Viscum album
Lymphocyte
T cell
Immunology
Lymphocyte proliferation
Natural killer cell
Immunomodulation
Mice
Immune system
Cell Movement
Stress, Physiological
Internal medicine
medicine
Animals
Immunology and Allergy
Lymphocytes
Cells, Cultured
Swimming
B cell
Cell Proliferation
Pharmacology
Mice, Inbred ICR
biology
Interleukin-6
Macrophages
Immobility Response, Tonic
biology.organism_classification
Molecular biology
Cold Temperature
Mice, Inbred C57BL
medicine.anatomical_structure
Endocrinology
Concanavalin A
biology.protein
Plant Lectins
Interleukin-1
Subjects
Details
- ISSN :
- 15675769
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- International Immunopharmacology
- Accession number :
- edsair.doi.dedup.....bf612ea664b6c24c05106d107b4416d6