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Violacein improves recombinant IgG production by controlling the cell cycle of Chinese hamster ovary cells
- Source :
- Cytotechnology
- Publication Year :
- 2020
- Publisher :
- Springer Science and Business Media LLC, 2020.
-
Abstract
- Chinese hamster ovary (CHO) cells are used as host cells for industrial monoclonal antibody (mAb) production. Cell cycle control is an effective approach to increase mAb production in the cell culture. Violacein, a purple-colored pigment produced by microorganisms, has diverse bioactive properties and has been proposed for various industrial applications. In this study, we evaluated the potency of violacein for cell cycle control and improvement of recombinant immunoglobulin G (IgG) production in CHO cells. Compared with the control, 0.9 μM violacein in a 14-day fed-batch culture increased the maximum IgG concentration by 37.6% via increasing the specific production rate and cell longevity. Cell cycle analysis showed that violacein induced G1 and G2/M phase arrest. However, the G1 arrest was observed only on day 1, while G2/M arrest lasted more than 3 days, suggesting that G2/M arrest mediated the violacein-induced enhanced IgG production. Moreover, in line with the increased protein expression, the expression levels of IgG mRNA and nutrient metabolic rates were also increased. N-Linked glycosylation and charge variant profiles were barely affected by violacein treatment. Our results indicate that violacein affects the cell cycle of CHO cells and increases IgG production without changing product quality, showing promise as a mAb production enhancer in CHO cells. The study provides insight into violacein utilization in industrial mAb manufacturing and can help develop advanced, effective mAb production technologies using CHO cell cultures.
- Subjects :
- 0301 basic medicine
Glycosylation
medicine.drug_class
Clinical Biochemistry
Biomedical Engineering
Bioengineering
Monoclonal antibody
Immunoglobulin G
law.invention
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
law
medicine
Messenger RNA
biology
Chinese hamster ovary cell
Cell Biology
Cell cycle
Molecular biology
030104 developmental biology
chemistry
Cell culture
030220 oncology & carcinogenesis
biology.protein
Recombinant DNA
Original Article
Biotechnology
Subjects
Details
- ISSN :
- 15730778 and 09209069
- Volume :
- 73
- Database :
- OpenAIRE
- Journal :
- Cytotechnology
- Accession number :
- edsair.doi.dedup.....bf2309570ca1fe1e3fabd2ff480b1dd6