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Circular mitochondrial DNA: A Geant4-DNA user application for evaluating radiation-induced damage in circular mitochondrial DNA
- Source :
- Journal of Medical Signals and Sensors, Journal of Medical Signals and Sensors, Vol 7, Iss 4, Pp 213-219 (2017)
- Publication Year :
- 2017
- Publisher :
- Medknow, 2017.
-
Abstract
- Background The aim of this study was to develop a nucleotide geometrical model of the circular mitochondrial DNA (mt-DNA) structure using Geant4-DNA toolkit to predict the radiation-induced damages such as single-strand breaks (SSB), double-strand breaks (DSB), and some other physical parameters. Methods Our model covers the organization of a circular human mt genetic system. The current model includes all 16,659 base pairs of human mt-DNA. This new mt-DNA model has been preliminarily tested in this work by determining SSB and DSB DNA damage yields and site-hit probabilities due to the impact of proton particles. The accuracy of the geometry was determined by three-dimensional visualization in various ring element numbers. The hit locations were determined with respect to a reference coordinate system, and the corresponding base pairs were stored in the ROOT output file. Results The coordinate determination according to the algorithm was consistent with the expected results. The output results contain the information about the energy transfers in the backbone region of the DNA double helix. The output file was analyzed by root analyzing tools. Estimation of SSBs and DSBs yielded similar results with the increment of incident particle linear energy transfer. In addition, these values seem to be consistent with the corresponding experimental determinations. Conclusions This model can be used in numerical simulations of mt-DNA radiation interactions to perform realistic evaluations of DNA-free radical reactions. This work will be extended to supercoiled conformation in the near future.
- Subjects :
- 0301 basic medicine
Work (thermodynamics)
Mitochondrial DNA
lcsh:Medical technology
DNA damage
Base pair
Coordinate system
Monte Carlo method
Biomedical Engineering
Geant4
Linear energy transfer
Health Informatics
geometrical model
030218 nuclear medicine & medical imaging
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Computer Science (miscellaneous)
Radiology, Nuclear Medicine and imaging
Monte Carlo
Genetics
Physics
Radiological and Ultrasound Technology
radiation
030104 developmental biology
lcsh:R855-855.5
chemistry
mitochondrial-DNA
Original Article
Biological system
DNA
Subjects
Details
- ISSN :
- 22287477
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- Journal of Medical Signals & Sensors
- Accession number :
- edsair.doi.dedup.....bf1c84fc9a9464f8f6091aa2ffd4232b