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Single-shot immunization with recombinant adenovirus encoding vaccinia virus glycoprotein A27L is protective against a virulent respiratory poxvirus infection
- Source :
- Vaccine. 28:4997-5004
- Publication Year :
- 2010
- Publisher :
- Elsevier BV, 2010.
-
Abstract
- Significant safety issues have emerged concerning the general use of DRYVAX® vaccine. Vaccination with replication-defective recombinant adenovirus (rAd) vaccines may offer a safer and effective alternative to live vaccinia virus (VV) vaccination. Six individual poxvirus glycoproteins: A33R, A34R, A36R, B5R, A27L or L1R that are normally expressed on the surface of infectious vaccinia virus were encoded in rAd vaccines and tested in mice in this study. A single-shot intramuscular injection of rAd encoding A27L protected mice against a lethal intranasal challenge with VV at 4 weeks post vaccination. By 10 weeks post vaccination, a significant decrease in post-challenge morbidity was observed that correlated with potent neutralizing antibody responses and the emergence of specific polyfunctional T cell responses. The immunogenicity and protective efficacy of rAd-A27L immunization persisted for at least 35 weeks post-vaccination. This study is the first demonstration that a single-shot subunit vaccine encoding a poxvirus protein confers protection against the mortality and morbidity associated with poxvirus infection.
- Subjects :
- CD4-Positive T-Lymphocytes
viruses
Vaccinia virus
Biology
Antibodies, Viral
Recombinant virus
Injections, Intramuscular
Article
Adenoviridae
Mice
Viral Proteins
chemistry.chemical_compound
Neutralization Tests
Animals
Poxviridae
Orthopoxvirus
Smallpox vaccine
Neutralizing antibody
Respiratory Tract Infections
Mice, Inbred BALB C
Vaccines, Synthetic
General Veterinary
General Immunology and Microbiology
Public Health, Environmental and Occupational Health
biology.organism_classification
Virology
Vaccination
Infectious Diseases
Immunization
chemistry
Antibody Formation
biology.protein
Molecular Medicine
Female
Vaccinia
Smallpox Vaccine
Spleen
Smallpox
Subjects
Details
- ISSN :
- 0264410X
- Volume :
- 28
- Database :
- OpenAIRE
- Journal :
- Vaccine
- Accession number :
- edsair.doi.dedup.....bf1696ff4abfa24193f3e890043cb94a