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The Insect Attractant 1-Octen-3-ol Is the Natural Ligand of Bovine Odorant-binding Protein
- Source :
- Journal of Biological Chemistry, Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2001, 276 (10), pp.7150-7155. ⟨10.1074/jbc.M010368200⟩
- Publication Year :
- 2001
- Publisher :
- HAL CCSD, 2001.
-
Abstract
- Bovine odorant-binding protein (bOBP) is a dimeric lipocalin present in large amounts in the respiratory and olfactory nasal mucosa. The structure of bOBP refined at 2.0-A resolution revealed an elongated volume of electron density inside each buried cavity, indicating the presence of one (or several) naturally occurring copurified ligand(s) (Tegoni et al. (1996) Nat. Struct. Biol. 3, 863-867; Bianchet et al. (1996) Nat. Struct. Biol. 3, 934-939). In the present work, by combining mass spectrometry, x-ray crystallography (1.8-A resolution), and fluorescence, it has been unambiguously established that natural bOBP contains the racemic form of 1-octen-3-ol. This volatile substance is a typical component of bovine breath and in general of odorous body emanations of humans and animals. The compound 1-octen-3-ol is also an extremely potent olfactory attractant for many insect species, including some parasite vectors like Anopheles (Plasmodium) or Glossina (Trypanosoma). For the first time, a function can be assigned to an OBP, with a possible role of bOBP in the ecological relationships between bovine and insect species.
- Subjects :
- Models, Molecular
Octanols
Insecta
Plasma protein binding
Lipocalin
Crystallography, X-Ray
Ligands
Receptors, Odorant
Biochemistry
Gas Chromatography-Mass Spectrometry
Mass Spectrometry
03 medical and health sciences
chemistry.chemical_compound
Animals
[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology
Binding site
Molecular Biology
ComputingMilieux_MISCELLANEOUS
Fluorescent Dyes
030304 developmental biology
0303 health sciences
Binding Sites
Dose-Response Relationship, Drug
biology
[SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM]
Ligand
030302 biochemistry & molecular biology
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology
Cell Biology
biology.organism_classification
3. Good health
[SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics
Kinetics
Spectrometry, Fluorescence
chemistry
1-Octen-3-ol
Trypanosoma
Odorant-binding protein
biology.protein
Cattle
Function (biology)
Subjects
Details
- Language :
- English
- ISSN :
- 00219258 and 1083351X
- Database :
- OpenAIRE
- Journal :
- Journal of Biological Chemistry, Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2001, 276 (10), pp.7150-7155. ⟨10.1074/jbc.M010368200⟩
- Accession number :
- edsair.doi.dedup.....bf115051c0e8cbd9704df0201ee79818
- Full Text :
- https://doi.org/10.1074/jbc.M010368200⟩