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First detection of multiresistance pRE25-like elements from Enterococcus spp. in Staphylococcus pseudintermedius isolated from canine pyoderma
- Source :
- Journal of Global Antimicrobial Resistance, Vol 20, Iss, Pp 304-308 (2020)
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- Objectives Enterococcus pRE25 is a conjugative and mobilising multiresistance plasmid from Enterococcus faecalis RE25. pRE25-like enterococcal plasmid pWZ909 mediates the delivery of vancomycin resistance to methicillin-resistant Staphylococcus aureus via a Tn1546-like transposon. However, whether pRE25-like elements contribute to multidrug resistance in Staphylococcus spp. has not yet been investigated. Here we describe the first detection of multiresistance pRE25-like elements in the chromosomal DNA of multidrug-resistant Staphylococcus pseudintermedius (MDRSP). Methods A total of 46 MDRSP clinical strains were isolated from canine pyoderma in Korea. Their genetic characteristics were analysed by multilocus sequence typing (MLST) and PCR targeting pRE25-like elements. Whole-genome sequencing (WGS) was performed on four isolates. Results WGS detected that the chromosomal 22-kb pRE25-like elements contained five antimicrobial resistance genes [cat, erm(B), aphA-3, aadK and sat4], IS1252, IS256, and a toxin–antitoxin system within copies of IS1216. BLASTn alignment analysis revealed that 84% of the chromosomal 22-kb pRE25-like elements sequence is homologous (99.8% identity) to the enterococcal pRE25 plasmid sequence. PCR assay showed that 52.2% of MDRSP isolates were positive for pRE25-like elements and were presumed to contain pRE25-like elements (pRE25 group). The sequence types (STs) of the pRE25 group were diverse, with 18 STs identified, among which 12 STs were first reported in Korea. Conclusion Enterococcal pRE25-like elements are suspected to be widespread in MDRSP isolated from companion dogs in Korea. Considering that companion dogs live in a closely shared environment with humans, continuous surveillance of pRE25-like elements is needed for other staphylococci commonly isolated from humans.
- Subjects :
- 0301 basic medicine
Microbiology (medical)
Staphylococcus pseudintermedius
Staphylococcus
030106 microbiology
Immunology
pRE25-like elements
Microbial Sensitivity Tests
Multidrug resistance
medicine.disease_cause
Microbiology
Enterococcus faecalis
03 medical and health sciences
Dogs
0302 clinical medicine
Plasmid
Bacterial Proteins
Drug Resistance, Multiple, Bacterial
Sequence Homology, Nucleic Acid
Republic of Korea
medicine
Animals
Immunology and Allergy
030212 general & internal medicine
Whole Genome Sequencing
biology
Vancomycin Resistance
Chromosomes, Bacterial
Staphylococcal Infections
biology.organism_classification
QR1-502
Interspersed Repetitive Sequences
Multiple drug resistance
Pyoderma
Enterococcus
Staphylococcus aureus
Multilocus sequence typing
Methicillin Resistance
Multilocus Sequence Typing
Plasmids
Subjects
Details
- ISSN :
- 22137165
- Volume :
- 20
- Database :
- OpenAIRE
- Journal :
- Journal of Global Antimicrobial Resistance
- Accession number :
- edsair.doi.dedup.....bf0cf5c61d56c824c9116f5d8b2cd998