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Cotranslational Intersection between the SRP and GET Targeting Pathways to the Endoplasmic Reticulum of Saccharomyces cerevisiae
- Source :
- Molecular and Cellular Biology. 36:2374-2383
- Publication Year :
- 2016
- Publisher :
- Informa UK Limited, 2016.
-
Abstract
- Targeting of transmembrane proteins to the endoplasmic reticulum (ER) proceeds via either the signal recognition particle (SRP) or the guided entry of tail-anchored proteins (GET) pathway, consisting of Get1 to -5 and Sgt2. While SRP cotranslationally targets membrane proteins containing one or multiple transmembrane domains, the GET pathway posttranslationally targets proteins containing a single C-terminal transmembrane domain termed the tail anchor. Here, we dissect the roles of the SRP and GET pathways in the sorting of homologous, two-membrane-spanning K(+) channel proteins termed Kcv, Kesv, and Kesv-VV. We show that Kcv is targeted to the ER cotranslationally via its N-terminal transmembrane domain, while Kesv-VV is targeted posttranslationally via its C-terminal transmembrane domain, which recruits Get4-5/Sgt2 and Get3. Unexpectedly, nascent Kcv recruited not only SRP but also the Get4-5 module of the GET pathway to ribosomes. Ribosome binding of Get4-5 was independent of Sgt2 and was strongly outcompeted by SRP. The combined data indicate a previously unrecognized cotranslational interplay between the SRP and GET pathways.
- Subjects :
- 0301 basic medicine
Potassium Channels
Saccharomyces cerevisiae Proteins
Transcription, Genetic
Saccharomyces cerevisiae
Biology
Endoplasmic Reticulum
environment and public health
Ribosome
03 medical and health sciences
Protein biosynthesis
Molecular Biology
Signal recognition particle receptor
Signal recognition particle
Endoplasmic reticulum
Articles
Cell Biology
Transmembrane protein
Cell biology
Transmembrane domain
030104 developmental biology
Membrane protein
Protein Biosynthesis
Carrier Proteins
Ribosomes
Signal Recognition Particle
Subjects
Details
- ISSN :
- 10985549
- Volume :
- 36
- Database :
- OpenAIRE
- Journal :
- Molecular and Cellular Biology
- Accession number :
- edsair.doi.dedup.....bf038e85e4d989677818fc059f1a41a7