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Different growth control of the two human thyroid cell lines of adenomatous goiter and papillary carcinoma
- Source :
- Thyroid : official journal of the American Thyroid Association. 5(1)
- Publication Year :
- 1995
-
Abstract
- To study the growth control of human thyroid cells in different stages of differentiation, we established two human thyroid cell lines of adenomatous goiter and papillary carcinoma. A 59-year-old female patient with adenomatous goiter was operated in September 1991, and a 27-year-old female patient with papillary carcinoma in May 1990. The thyroid cell lines were established by successive passage without cellular or genetic manipulations such as fusing other cell lines or oncogenic viral infection. These cell lines, human adenomatous goiter cells (hAG) and human papillary thyroid carcinoma cells (hPTC), exhibited a flattened polygonal shape and proliferated as a monolayer in cell culture. The doubling time of the hAG cells was 60 h in Ham's F12 medium supplemented with 10% fetal bovine serum, and that of the hPTC cells, 18 h in the same medium. Both cell lines expressed mRNA for TSH receptor and secreted cAMP into the medium during incubation with thyrotropin (TSH) at concentrations as low as 0.01 mU/mL. The effects of activators of protein kinase A (PKA), protein kinase C (PKC), tyrosine kinase (TK), and estradiol (E2) on proliferation of the hAG cells and the hPTC cells were assessed by measuring cellular DNA content in 24-well plates with diaminobenzoic acid. TSH stimulated proliferation of the hAG cells, but it inhibited proliferation of the hPTC cells. Since TSH activates two signaling pathways, the adenyl cyclase-PKA system and phospholipase C-PKC system, we tested effects of dibutylyl cAMP (dBC) and phorbol myristate 13-acetate (PMA), separately. dBC stimulated proliferation of the hAG cells, but it inhibited that of the hPTC cells.(ABSTRACT TRUNCATED AT 250 WORDS)
- Subjects :
- Adenoma
Adult
endocrine system
medicine.medical_specialty
endocrine system diseases
Cell division
Endocrinology, Diabetes and Metabolism
Thyrotropin
Thyroid carcinoma
Endocrinology
Internal medicine
medicine
Cyclic AMP
Tumor Cells, Cultured
Humans
RNA, Messenger
Thyroid Neoplasms
Protein kinase A
Growth Substances
Protein kinase C
Estradiol
business.industry
Goiter
Phosphotransferases
Receptors, Thyrotropin
DNA, Neoplasm
Middle Aged
Molecular biology
Carcinoma, Papillary
Bucladesine
Cell culture
Tetradecanoylphorbol Acetate
Female
Signal transduction
business
Tyrosine kinase
Fetal bovine serum
Cell Division
Subjects
Details
- ISSN :
- 10507256
- Volume :
- 5
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Thyroid : official journal of the American Thyroid Association
- Accession number :
- edsair.doi.dedup.....befeae888da4086150be846261027294