Back to Search
Start Over
CD25+ cell depletion hastens the onset of severe disease in collagen-induced arthritis
- Source :
- Arthritis and Rheumatism, 48, 5, pp. 1452-60, Arthritis and rheumatism, 48(5), 1452-1460. John Wiley and Sons Inc., Arthritis and Rheumatism, 48, 1452-60
- Publication Year :
- 2003
-
Abstract
- Item does not contain fulltext OBJECTIVE: CD4+,CD25+ T regulatory cells may offer opportunities to intervene in the course of autoimmune disease. We wished to evaluate their potential for influencing systemic and chronic joint inflammation by investigating their involvement in collagen-induced arthritis (CIA). METHODS: We depleted DBA/1 mice of CD25+ regulatory cells by injection of a depleting monoclonal antibody specific for CD25 14 days before a single immunization with type II collagen (CII) in Freund's complete adjuvant. CD4+,CD25+ T cells were adoptively transferred to some groups of mice during immunization. Mice were then scored for signs of arthritis, and blood was taken periodically to measure the amounts of CII-specific antibodies. Splenocytes of treated mice were examined in vitro to determine the effects of depletion on proliferation to CII and control antigens. RESULTS: CD25+ cell-depleted DBA/1 mice had significantly more severe disease than control mice following collagen immunization. The magnified severity was also accompanied by higher antibody titers against collagen, and in vitro tests showed increased proliferation of collagen-specific T cells. Adoptively transferring CD4+,CD25+ T cells into depleted mice was shown to reverse the heightened severity. Control mice, which were depleted and immunized with the neoantigen keyhole limpet hemocyanin (KLH), had neither an increased antibody response toward KLH nor an augmented proliferative response, indicating that CD25+ cell depletion preferentially affects immunity against self antigen. CONCLUSION: These results establish a link between CD4+,CD25+ regulatory cells and CIA and provide a rationale for investigating CD4+,CD25+ T regulatory cells in the treatment and prevention of arthritis.
- Subjects :
- Male
Adoptive cell transfer
medicine.drug_class
T-Lymphocytes
Immunology
Freund's Adjuvant
Arthritis
Enzyme-Linked Immunosorbent Assay
chemical and pharmacologic phenomena
Monoclonal antibody
Lymphocyte Depletion
Mice
Rheumatology
Antigen
Forelimb
medicine
Immunology and Allergy
Animals
Edema
Pharmacology (medical)
IL-2 receptor
Collagen Type II
biology
business.industry
Antibodies, Monoclonal
Receptors, Interleukin-2
hemic and immune systems
medicine.disease
Flow Cytometry
Adoptive Transfer
Arthritis, Experimental
Hindlimb
Rats
Tumor microenvironment [UMCN 1.3]
Freund's adjuvant
Mice, Inbred DBA
CD4 Antigens
Hemocyanins
biology.protein
Immunization
Antibody
business
Keyhole limpet hemocyanin
Cell Division
Spleen
Subjects
Details
- ISSN :
- 00043591
- Database :
- OpenAIRE
- Journal :
- Arthritis and Rheumatism, 48, 5, pp. 1452-60, Arthritis and rheumatism, 48(5), 1452-1460. John Wiley and Sons Inc., Arthritis and Rheumatism, 48, 1452-60
- Accession number :
- edsair.doi.dedup.....bed62f0a889858e9987e5d2707207477