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Optical pH measurement system using a single fluorescent dye for assessing susceptibility to dental caries
- Source :
- Journal of Biomedical Optics
- Publication Year :
- 2019
- Publisher :
- SPIE-Intl Soc Optical Eng, 2019.
-
Abstract
- Sugar-rich diets and poor dental hygiene promote the formation of a biofilm (plaque) that strongly adheres to the dental enamel surface and fosters the evolution of aciduric bacteria. The acid contributes to demineralization of the exterior tooth enamel, which accelerates after the pH drops below a critical value (∼5.5) for extended time periods resulting in the need for restorative procedures. Preventative techniques to alert the dentist and caries-susceptible patients regarding vulnerability to dental decay require a clinical measure of plaque activity. Therefore, there is a need to evaluate the acid production capability of plaque deposits in the pits and fissures of occlusal and interproximal regions. A ratiometric fluorescence pH-sensing device has been developed using an FDA-approved dye and LED excitation. Fluorescein spectral profiles were collected using a spectrometer and analyzed with a spectral unmixing algorithm for calibration over the pH range of 4.5 to 7. An in vivo pilot study on human subjects was performed using a sucrose rinse to accelerate bacterial metabolism and to measure the time-dependent drop in pH. The optical system is relatively immune to confounding factors such as photobleaching, dye concentration, and variation in excitation intensity associated with earlier dye-based pH measurement techniques.
- Subjects :
- Adult
Male
Paper
Optics and Photonics
Sucrose
fluorescein
Dental Plaque
Biomedical Engineering
Pilot Projects
Buffers
Dental Caries
plaque
Biomaterials
Young Adult
dianion
Humans
Dental Enamel
Tooth Demineralization
Fluorescent Dyes
caries
unmixing
Errata
pH
Stephan curve
anion
Hydrogen-Ion Concentration
Oral Hygiene
Atomic and Molecular Physics, and Optics
Electronic, Optical and Magnetic Materials
Spectrometry, Fluorescence
Biofilms
Calibration
Sensing
Female
fluorescence
Algorithms
Subjects
Details
- ISSN :
- 10833668
- Volume :
- 24
- Database :
- OpenAIRE
- Journal :
- Journal of Biomedical Optics
- Accession number :
- edsair.doi.dedup.....beb09bed2d69859d90155d6900f73589