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The efficacy of in vivo administration of Apremilast on mesenchymal stem cells derived from psoriatic patients
- Source :
- Inflammation Research. 70:79-87
- Publication Year :
- 2020
- Publisher :
- Springer Science and Business Media LLC, 2020.
-
Abstract
- Psoriasis cellular hallmarks, such as the imbalance between Th1/Th17 and Th2 cytokines and the dysregulated expression of vascular endothelial growth factor (VEGF), inducible nitric oxide synthase, (iNOS) and indoleamine 2,3-dioxygenase (IDO), are all detectable in mesenchymal stem cells (MSCs) suggesting that psoriasis originates at mesenchymal level. Aim of the study: In this scenario, MSCs may become the new therapeutic target and interest in the effects of traditionally used drugs, such as Apremilast, on MSCs has greatly increased. MSCs from control subjects (C-MSCs) and from psoriatic patients before (PsO MSCs T0) and after in vivo treatment with Apremilast (PsO-MSCs T12) were isolated and characterized; subsequently, the effects of Apremilast on VEGF, iNOS and IDO expression were evaluated by immunocytochemistry (ICC). The expression of VEGF, iNOS and IDO was also detected in skin sections by immunohistochemistry (IHC). The results indicate that in vivo administration of Apremilast is able to drive the altered profile of PsO-MSCs towards a more physiological pattern. In skin sections, the role of Apremilast is evident in reducing VEGF, iNOS and IDO expression. Apremilast treatment influences the expression of VEGF, iNOS and IDO not only by keratinocytes but also by MSCs, restoring their intrinsic profile and their natural anti-inflammatory action, and decreasing the auto-inflammatory process that underpins the development of psoriasis.
- Subjects :
- Adult
Male
Vascular Endothelial Growth Factor A
0301 basic medicine
Immunology
Nitric Oxide Synthase Type II
Inflammation
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
In vivo
Psoriasis
medicine
Humans
Indoleamine-Pyrrole 2,3,-Dioxygenase
Aged
Skin
Pharmacology
biology
business.industry
Anti-Inflammatory Agents, Non-Steroidal
Mesenchymal stem cell
Mesenchymal Stem Cells
Middle Aged
medicine.disease
Thalidomide
Nitric oxide synthase
Vascular endothelial growth factor
030104 developmental biology
chemistry
030220 oncology & carcinogenesis
biology.protein
Cancer research
Immunohistochemistry
Female
Apremilast
medicine.symptom
business
medicine.drug
Subjects
Details
- ISSN :
- 1420908X and 10233830
- Volume :
- 70
- Database :
- OpenAIRE
- Journal :
- Inflammation Research
- Accession number :
- edsair.doi.dedup.....bea8686aad106e08a846d5ac414de6d1