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Determination and optimization of a strong promoter element from Bacillus amyloliquefaciens by using a promoter probe vector
- Source :
- Biotechnology letters. 40(1)
- Publication Year :
- 2017
-
Abstract
- To construct a promoter probe vector, pBE-bgaB, to screen strong promoters from Bacillus amyloliquefaciens. 266 colonies containing active promoter elements from the genomic DNA of B. amyloliquefaciens were identified. Among these, promoter P41 exhibited the strongest β-Gal activity in Escherichia coli and B. amyloliquefaciens. Sequence analysis showed that promoter P41 contained P ykuN , a ykuN gene encoding flavodoxin. Optimization of the ribosome-binding site from P41 to P382 improved β-Gal activity by ~ 200%. A new strong promoter for protein expression and genetic engineering of Bacillus species.
- Subjects :
- 0301 basic medicine
Bacillus amyloliquefaciens
Sequence analysis
Flavodoxin
030106 microbiology
Genetic Vectors
Bioengineering
medicine.disease_cause
Applied Microbiology and Biotechnology
03 medical and health sciences
Genes, Reporter
medicine
Escherichia coli
Genetic Testing
Promoter Regions, Genetic
Gene
biology
Chemistry
Promoter
General Medicine
biology.organism_classification
beta-Galactosidase
Molecular biology
Ribosomal binding site
Artificial Gene Fusion
genomic DNA
030104 developmental biology
biology.protein
Biotechnology
Subjects
Details
- ISSN :
- 15736776
- Volume :
- 40
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Biotechnology letters
- Accession number :
- edsair.doi.dedup.....be9dbdad0ae46cc94976dc3e855d5806