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Inhaled Gene Transfer for Pulmonary Circulation
- Source :
- Methods in Molecular Biology ISBN: 9781493965861
- Publication Year :
- 2016
- Publisher :
- Springer New York, 2016.
-
Abstract
- Chronic pulmonary hypertension (PH) is associated with right ventricular failure and high mortality regardless of the underlying disease. Currently, therapies can improve clinical outcomes in specific subsets of patients, but have little impact on the progression of pulmonary vascular remodeling. Upon new advances in vector development and delivery techniques, gene therapy is a novel strategy in this field with the potential of overcoming the main limitations of approved drug therapies: modulation of novel anti-remodeling targets and selective pulmonary vasculature targeting with minimal systemic effects. In the recent years, several reports have shown that gene transfer to the pulmonary vascular system is feasible in rodent models of PH. Our group has focused on the translation of airway delivery of viral vectors in small and large animals. Here, we describe a procedure to achieve vector transduction at the distal vasculature in animal models of PH and the methods to evaluate the outcomes of this intervention as a promising new approach in pulmonary vascular diseases.
- Subjects :
- 0301 basic medicine
Pulmonary Circulation
Swine
Heart Ventricles
Genetic enhancement
Gene transfer
030204 cardiovascular system & hematology
medicine.disease_cause
Bioinformatics
Approved drug
Article
Viral vector
Electrocardiography
03 medical and health sciences
Transduction (genetics)
0302 clinical medicine
Transduction, Genetic
Administration, Inhalation
medicine
Animals
Adeno-associated virus
business.industry
Gene Transfer Techniques
Hemodynamics
medicine.disease
Pulmonary hypertension
Disease Models, Animal
030104 developmental biology
business
Airway
Subjects
Details
- ISBN :
- 978-1-4939-6586-1
- ISBNs :
- 9781493965861
- Database :
- OpenAIRE
- Journal :
- Methods in Molecular Biology ISBN: 9781493965861
- Accession number :
- edsair.doi.dedup.....be9a990e1d9e09576515d3a9fbb0eb98