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Prebiotic and synbiotic fructooligosaccharide administration fails to reduce the severity of experimental colitis in rats
- Publication Year :
- 2007
- Publisher :
- United States : Lippincott Williams & Wilkins, 2007.
-
Abstract
- Opposing effects of the prebiotic, fructooligosaccharide, have been reported in experimental colitis. We compared the effects of the prebiotic, fructooligosaccharide, alone and in synbiotic combination with Lactobacillus fermentum BR11, on the development of dextran sulfate sodium-induced colitis in rats. Rats consumed an 18 percent casein-based diet or diet supplemented with 6 percent fructooligosaccharide or maltodextrin for 14 days. The synbiotic group was gavaged 1 ml of L. fermentum BR11 (1 × 109 cfu/ml) twice daily. From Days 7 to 14, colitis was induced via 3 percent dextran sulfate sodium in drinking water. Disease activity was assessed daily, and at killing, gastrointestinal organs were measured, weighed, and examined by quantitative histology, proliferating cell nuclear antigen immunohistochemistry, and colonic myeloperoxidase activity. Administration of dextran sulfate sodium resulted in an increased colitic disease activity, and an increased colon and cecum weight compared with normal controls. Colon and cecum weights were further increased in dextran sulfate sodium+fructooligosaccharide (colon: 19 percent; cecum: 48 percent) and dextran sulfate sodium+fructooligosaccharide/L. fermentum BR11-treated rats (16 and 62 percent) compared with dextran sulfate sodium+vehicle-treatment. Dextran sulfate sodium+fructooligosaccharide-treated rats displayed an 81 percent increase in colonic myeloperoxidase activity compared with dextran sulfate sodium-treated controls. Histologic damage severity scores increased in dextran sulfate sodium+vehicle, dextran sulfate sodium+fructooligosaccharide, and dextran sulfate sodium+fructooligosaccharide/L. fermentum BR11-treated rats compared with normal controls (P
- Subjects :
- Male
medicine.medical_specialty
Limosilactobacillus fermentum
Diet therapy
Lactobacillus fermentum
Colon
medicine.medical_treatment
Clinical Sciences
Administration, Oral
Oligosaccharides
Severity of Illness Index
Microbiology
Rats, Sprague-Dawley
chemistry.chemical_compound
Cecum
coon
inflammatory bowel disease
Internal medicine
Sodium sulfate
medicine
Animals
fructooligosaccharide
Treatment Failure
Colitis
Cell Proliferation
Peroxidase
biology
business.industry
Fructooligosaccharide
Prebiotic
Probiotics
Dextran Sulfate
Gastroenterology
General Medicine
medicine.disease
biology.organism_classification
Rats
lactobacillus
Disease Models, Animal
Dextran
Endocrinology
medicine.anatomical_structure
chemistry
dextran sulfate sodium
business
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....be9a467a4f5f7bba8dd643f40b773135