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Silver Nanoparticles Biosynthesized with Spruce Bark Extract—A Molecular Aggregate with Antifungal Activity against Candida Species
- Source :
- Antibiotics, Antibiotics, Vol 10, Iss 1261, p 1261 (2021), Volume 10, Issue 10
- Publication Year :
- 2021
- Publisher :
- MDPI, 2021.
-
Abstract
- Due to their high content of biomolecules, combined with silver’s well known antimicrobial potential, silver nanoparticles biosynthesized using spruce bark (AgNP SBEs) demonstrate antibacterial and antioxidant activity, making them a versatile option for developing new antimicrobial agents that might be used for medical treatment or as adjuvants for the classical agents. This study aims to analyze if silver nanoparticles (AgNPs) mediated by spruce bark extract (SBE) and silver salts (AgNP SBE Acetate, AgNP SBE Nitrate) presents antifungal activity against five different Candida spp., synergistic activity with fluconazole, and if they interact with some virulence factors of C. albicans. AgNP SBEs presented MICs (minimum inhibitory concentrations) for all the five tested Candida spp. AgNP SBEs inhibited the growth of C. parapsilosis, C. krusei, and C. guilliermondii, exerted synergistic activity with fluconazole for C. parapsilosis and C. guilliermondii, and inhibited biofilm production for C. albicans, C. auris, and C. guilliermondii. MICs of AgNP SBE Acetate significantly inhibited the production of germ tubes of C. albicans. The expression of C. albicans&nbsp<br />SAP2 gene was down-regulated by the short-time treatment with MICs of AgNP SBE Acetate, while ALS3 and HSP70 genes were up-regulated by the AgNPs MICs. These results emphasize the potential of using the AgNP SBEs as treatments/adjuvants options, not only against the redundant C. albicans but also for the non-albicans Candida species (which are not as frequently involved in human pathologies, but, sometimes, can be more aggressive).
- Subjects :
- Microbiology (medical)
silver nanoparticles
SAP2
Antioxidant
HSP70
ALS3
medicine.medical_treatment
Germ tube
RM1-950
Biochemistry
Microbiology
Silver nanoparticle
Article
biofilm
synergism
medicine
Pharmacology (medical)
General Pharmacology, Toxicology and Pharmaceutics
Chemistry
Biofilm
spruce bark
Antimicrobial
Corpus albicans
Infectious Diseases
visual_art
visual_art.visual_art_medium
Bark
Therapeutics. Pharmacology
biosynthesis
Fluconazole
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 20796382
- Volume :
- 10
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- Antibiotics
- Accession number :
- edsair.doi.dedup.....be9334c9ddfce9506fa6e5852c8bc3e0