Back to Search
Start Over
Apoptosis and schizophrenia: a pilot study based on dermal fibroblast cell lines
- Source :
- Schizophrenia research. 84(1)
- Publication Year :
- 2005
-
Abstract
- Introduction The aim of this study was to investigate whether there is an increased susceptibility to apoptosis in cultured fibroblasts from patients with schizophrenia. Method Dermal fibroblasts were collected and cultured from three groups: patients with schizophrenia, patients with non-schizophrenic psychosis, and healthy comparison subjects. Susceptibility to apoptosis was measured at the level of degradation product (proportion of cells in the sub-G0 cell cycle fraction in which apoptotic bodies accumulate), pro-apoptotic effector (activated caspase-3), and molecular regulators (P53, Bax and Bcl-2). Cell lines were studied under both basal culture and cycloheximide (an apoptotic inducer) exposure conditions. Results Consistent with increased susceptibility to apoptosis, the proportion of sub-G0 cells under basal conditions was significantly larger in the schizophrenia group, compared to the non-schizophrenic psychosis group. However when apoptosis was stimulated with cycloheximide, the schizophrenia group showed an attenuated caspase-3 response. The pattern of correlations between regulators, caspase-3 and the proportion of sub-G0 cells was different in the schizophrenia group, consistent with group-specific apoptotic pathway dysregulation. Conclusion The study demonstrated anomalous apoptotic mechanisms in schizophrenia, which appear not to affect non-schizophrenia psychosis patients. The detection of these anomalies in fibroblasts suggests that altered apoptosis may be observable in all somatic cell types in schizophrenia.
- Subjects :
- Adult
Male
medicine.medical_specialty
Psychosis
Antifungal Agents
Somatic cell
Apoptosis
Pilot Projects
Cycloheximide
Cell Line
Dermal fibroblast
chemistry.chemical_compound
Internal medicine
mental disorders
medicine
Humans
Fibroblast
Biological Psychiatry
Cells, Cultured
business.industry
Caspase 3
Cell Cycle
Dermis
Cell cycle
Fibroblasts
medicine.disease
Flow Cytometry
Psychiatry and Mental health
medicine.anatomical_structure
Endocrinology
chemistry
Psychotic Disorders
Cell culture
Caspases
Schizophrenia
Female
business
Neuroscience
Subjects
Details
- ISSN :
- 09209964
- Volume :
- 84
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Schizophrenia research
- Accession number :
- edsair.doi.dedup.....be7da60f2ebb419bb86f34782abe4c6f