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Production of Itaconic Acid from Cellulose Pulp: Feedstock Feasibility and Process Strategies for an Efficient Microbial Performance
- Source :
- Energies, Volume 13, Issue 7, Pages: 1654, Kerssemakers, A A J, Doménech, P, Cassano, M, Yamakawa, C K, Dragone, G & Mussatto, S I 2020, ' Production of itaconic acid from cellulose pulp: Feedstock feasibility and process strategies for an efficient microbial performance ', Energies, vol. 13, no. 7, en13071654 . https://doi.org/10.3390/en13071654, Energies, Vol 13, Iss 1654, p 1654 (2020)
- Publication Year :
- 2020
- Publisher :
- MDPI AG, 2020.
-
Abstract
- This study assessed the feasibility of using bleached cellulose pulp from Eucalyptus wood as a feedstock for the production of itaconic acid by fermentation. Additionally, different process strategies were tested with the aim of selecting suitable conditions for an efficient production of itaconic acid by the fungus Aspergillus terreus. The feasibility of using cellulose pulp was demonstrated through assays that revealed the preference of the strain in using glucose as carbon source instead of xylose, mannose, sucrose or glycerol. Additionally, the cellulose pulp was easily digested by enzymes without requiring a previous step of pretreatment, producing a glucose-rich hydrolysate with a very low level of inhibitor compounds, suitable for use as a fermentation medium. Fermentation assays revealed that the technique used for sterilization of the hydrolysate (membrane filtration or autoclaving) had an important effect in its composition, especially on the nitrogen content, consequently affecting the fermentation performance. The carbon-to-nitrogen ratio (C:N ratio), initial glucose concentration and oxygen availability, were also important variables affecting the performance of the strain to produce itaconic acid from cellulose pulp hydrolysate. By selecting appropriate process conditions (sterilization by membrane filtration, medium supplementation with 3 g/L (NH4)2SO4, 60 g/L of initial glucose concentration, and oxygen availability of 7.33 (volume of air/volume of medium)), the production of itaconic acid was maximized resulting in a yield of 0.62 g/g glucose consumed, and productivity of 0.52 g/L·h.
- Subjects :
- 0106 biological sciences
Control and Optimization
Sucrose
020209 energy
Energy Engineering and Power Technology
Lignocellulosic biomass
02 engineering and technology
Xylose
lcsh:Technology
01 natural sciences
Hydrolysate
cellulose pulp
C:N ratio
chemistry.chemical_compound
Hydrolysis
010608 biotechnology
0202 electrical engineering, electronic engineering, information engineering
oxygen availability
Aspergillus terreus
Itaconic acid
Food science
Electrical and Electronic Engineering
fermentation
Engineering (miscellaneous)
lignocellulosic biomass
biorefinery
biology
lcsh:T
Renewable Energy, Sustainability and the Environment
Chemistry
itaconic acid
biology.organism_classification
hydrolysis
Fermentation
Energy (miscellaneous)
Subjects
Details
- ISSN :
- 19961073
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- Energies
- Accession number :
- edsair.doi.dedup.....be622d6b2152014f7279dabf89e32887