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Alterations in DNA methyltransferases and methyl-CpG binding domain proteins during cleft palate formation as induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in mice
- Source :
- Molecular Medicine Reports.
- Publication Year :
- 2018
- Publisher :
- Spandidos Publications, 2018.
-
Abstract
- Maternal exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) induces cleft palate formation in mice. This TCDD treatment, which may be considered an environmental factor in cleft palate formation, is associated with alterations in DNA methylation. However, the underlying molecular mechanisms of DNA methylation produced by TCDD in mouse embryos are poorly understood. DNA methyltransferases (DNMTs) and methyl‑CpG binding domain proteins (MBDs) are thought to be closely associated with the actions of DNA methylation. Therefore, the present study tested the hypothesis that this cleft palate inducing effect of TCDD will alter the expression levels of DNMTs and various MBDs in palate tissue of fetal mice. Pregnant C57BL/6J mice were treated with either TCDD (64 µg/kg) or corn oil (control) at embryonic day 10.5 (E10.5) and fetal palates were harvested for structural and molecular analyses at E13.5, E14.5, E15.5 and E17.5. Expression levels of DNMTs and MBDs were assayed using reverse transcription‑quantitative polymerase chain reaction and western blotting. The incidence of cleft palates in the TCDD group was 98.24%, whereas no cases of cleft palate were observed in the control group. Expression levels of DNMTs and MBDs were significantly increased in the TCDD group compared with the control. The results demonstrate clear alterations in DNMTs and MBDs, as induced by TCDD, and suggest that such alterations are important in cleft palate formation in fetal mice.
- Subjects :
- 0301 basic medicine
Cancer Research
Polychlorinated Dibenzodioxins
Methyltransferase
Biology
Biochemistry
DNA methyltransferase
Andrology
Mice
03 medical and health sciences
Pregnancy
Genetics
Animals
DNA (Cytosine-5-)-Methyltransferases
Molecular Biology
reproductive and urinary physiology
Regulation of gene expression
Gene Expression Regulation, Developmental
Genetic Variation
Embryo, Mammalian
Molecular medicine
Up-Regulation
Methyl-CpG-binding domain
Cleft Palate
DNA-Binding Proteins
Blot
Disease Models, Animal
030104 developmental biology
Oncology
embryonic structures
DNA methylation
Molecular Medicine
Female
Binding domain
Subjects
Details
- ISSN :
- 17913004 and 17912997
- Database :
- OpenAIRE
- Journal :
- Molecular Medicine Reports
- Accession number :
- edsair.doi.dedup.....be542cc3d0ae12c16d5c61f04845820e