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In vitro assessment of the efficacy of protein exudates from seeds against Haemonchus contortus
- Source :
- Veterinary Parasitology. 292:109399
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- Nematodes develop resistance to the most common commercially available drugs. The aim of this study was to identify and evaluate the action of protein exudates from Mimosa caesalpiniifolia, Leucaena leucocephala, Acacia mangium, and Stylosanthes capitata seeds on the gastrointestinal nematode Haemonchus contortus. The exuded proteins were precipitated, dialyzed, lyophilized, and assessed for their effect on egg hatching and artificial larval exsheathment inhibition. Proteome analysis of the protein extracts was also performed. Although no egg-hatching inhibition was observed, all exudates showed efficacy in inhibiting the larval exsheathment of H. contortus larvae with an EC50 varying from 0.61 to 0.26 mg P mL−1. Proteomic analysis revealed the presence of proteases, protease inhibitors, chitinases, and lectins among other proteins in the exudates. Most of the exuded proteins belong to the oxidative stress/plant defense and energy/carbohydrate metabolism functional clusters. This study concluded that the bioactive proteins from different classes exuded by seeds of M. caesalpiniifolia, L. leucocephala, A. mangium, and S. capitata show stage-specific inhibition against H. contortus.
- Subjects :
- 0301 basic medicine
Proteases
Plant Exudates
medicine.medical_treatment
030231 tropical medicine
Biology
Microbiology
03 medical and health sciences
0302 clinical medicine
Acacia mangium
parasitic diseases
Plant defense against herbivory
medicine
Animals
Plant Proteins
Anthelmintics
Leucaena leucocephala
Protease
General Veterinary
Fabaceae
Exudates and Transudates
General Medicine
030108 mycology & parasitology
biology.organism_classification
Nematode
Seeds
Proteome
Haemonchus
Parasitology
Haemonchus contortus
Subjects
Details
- ISSN :
- 03044017
- Volume :
- 292
- Database :
- OpenAIRE
- Journal :
- Veterinary Parasitology
- Accession number :
- edsair.doi.dedup.....be464ffd96b24832ae89b98b04512bfc