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Effects of hydrogen peroxide on biological characteristics and osteoinductivity of decellularized and demineralized bone matrices
- Source :
- Journal of Biomedical Materials Research Part A. 107:1476-1490
- Publication Year :
- 2019
- Publisher :
- Wiley, 2019.
-
Abstract
- Due to the similar collagen composition and closely physiological relationship with soft connective tissues, demineralized bone matrices (DBMs) were used to repair the injured tendon or ligament. However, the osteoinductivity of DBMs would be a huge barrier of these applications. Hydrogen peroxide (H2 O2 ) has been proved to reduce the osteoinductivity of DBMs. Nevertheless, the biological properties of H2 O2 -treated DBMs have not been evaluated completely, while the potential mechanism of H2 O2 compromising osteoinductivity is also unclear. Hence, the purpose of this study was to characterize the biological properties of H2 O2 -treated DBMs and search for the proof that H2 O2 could compromise osteoinductivity of DBMs. Decellularized and demineralized bone matrices (DCDBMs) were washed by 3% H2 O2 for 12 h to fabricate the H2 O2 -treated DCDBMs (HPTBMs). Similar biological properties including collagen, biomechanics, and biocompatibility were observed between DCDBMs and HPTBMs. The immunohistochemistry staining of bone morphogenetic protein 2 (BMP-2) was negative in HPTBMs. Furthermore, HPTBMs exhibited significantly reduced osteoinductivity both in vitro and in vivo. Taken together, these findings suggest that the BMP-2 in DCDBMs could be the target of H2 O2 . HPTBMs could be expected to be used as a promising scaffold for tissue engineering. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2019.
- Subjects :
- Male
Scaffold
Materials science
Biocompatibility
0206 medical engineering
Biomedical Engineering
Bone Matrix
02 engineering and technology
Bone morphogenetic protein 2
Rats, Sprague-Dawley
Biomaterials
Mice
Calcification, Physiologic
Tissue engineering
Osseointegration
Osteogenesis
In vivo
medicine
Animals
Decellularization
Metals and Alloys
Hydrogen Peroxide
021001 nanoscience & nanotechnology
020601 biomedical engineering
In vitro
Tendon
medicine.anatomical_structure
Gene Expression Regulation
NIH 3T3 Cells
Ceramics and Composites
Cattle
0210 nano-technology
Biomedical engineering
Subjects
Details
- ISSN :
- 15524965 and 15493296
- Volume :
- 107
- Database :
- OpenAIRE
- Journal :
- Journal of Biomedical Materials Research Part A
- Accession number :
- edsair.doi.dedup.....be3f07861d95e74cc03b48982f516a1c