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Impact of temperature dependent sampling procedures in proteomics and peptidomics--a characterization of the liver and pancreas post mortem degradome

Authors :
Kim Kultima
Karl Sköld
Robert Stella
Peter James
Birger Scholz
Mikhail M. Savitski
Sofia Waldemarson
Marcus Söderquist
Roman A. Zubarev
Per E. Andrén
Mats Borén
Céline Fernandez
Source :
Molecularcellular proteomics : MCP. 10(3)
Publication Year :
2010

Abstract

Little is known about the nature of post mortem degradation of proteins and peptides on a global level, the so-called degradome. This is especially true for nonneural tissues. Degradome properties in relation to sampling procedures on different tissues are of great importance for the studies of, for instance, post translational modifications and/or the establishment of clinical biobanks. Here, snap freezing of fresh (

Details

ISSN :
15359484
Volume :
10
Issue :
3
Database :
OpenAIRE
Journal :
Molecularcellular proteomics : MCP
Accession number :
edsair.doi.dedup.....bdfe06277a954e3c99a13a0dc6f2cb38