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Dried haematic microsamples and LC–MS/MS for the analysis of natural and synthetic cannabinoids
- Publication Year :
- 2017
-
Abstract
- Synthetic cannabinoids are new psychoactive substances (NPS) with similar effects when compared to natural ones found in Cannabis derivatives. They have rapidly integrated into the illicit market, often sold as alternatives under international control. The need to identify and quantify an unprecedented and growing number of new compounds represents a unique challenge for toxicological, forensic and anti-doping analysis. Dried blood spots have been used within the bioanalytical framework in place of plasma or serum, in order to reduce invasiveness, lower sample size, simplify handling, storage and shipping of samples and to facilitate home-based and on-field applications. However, DBS implementation has been limited mainly by concerns related to haematocrit effect on method accuracy. Volumetric absorptive microsampling (VAMS™), a second generation dried miniaturized sampling technology, has been developed just in order to eliminate haematocrit effect, thus providing accurate sampling but still granting feasible sample processing. An original LC-MS/MS method was herein developed and validated for the analysis of THC and its 2 main metabolites, together with 10 representative synthetic cannabinoids in both DBS and VAMS dried microsamples. The ultimate goal of this work is to provide highly innovative DBS and VAMS analytical protocols, whose performances were extensively optimized and compared, in order to provide effective and alternative tools that can be applied for natural and synthetic cannabinoid determination, in place of classical analytical strategies.
- Subjects :
- Bioanalysis
Sample processing
Clinical Biochemistry
Analytical chemistry
Dried blood spot (DBS)
030226 pharmacology & pharmacy
01 natural sciences
Biochemistry
Analytical Chemistry
03 medical and health sciences
0302 clinical medicine
Drug Stability
LC–MS/MS
Limit of Detection
Tandem Mass Spectrometry
Illicit market
Synthetic cannabinoids
Lc ms ms
medicine
Humans
Dried blood
Chromatography
Cannabis derivatives
Chemistry
Cannabinoids
010401 analytical chemistry
Reproducibility of Results
Natural cannabinoid
General Medicine
Cell Biology
0104 chemical sciences
Substance Abuse Detection
Linear Models
Volumetric absorptive microsampling (VAMS)
Biochemical engineering
Dried Blood Spot Testing
Synthetic cannabinoid
Blood sampling
medicine.drug
Chromatography, Liquid
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....bdd9e3c12b9304969079ce9d58f03b55