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High-resolution optical control of spatiotemporal neuronal activity patterns in zebrafish using a digital micromirror device

Authors :
Rainer W. Friedrich
Peixin Zhu
Jennifer Shum
Yan-Ping Zhang Schärer
Otto Fajardo
Source :
Nature Protocols. 7:1410-1425
Publication Year :
2012
Publisher :
Springer Science and Business Media LLC, 2012.

Abstract

Optogenetic approaches allow the manipulation of neuronal activity patterns in space and time by light, particularly in small animals such as zebrafish. However, most techniques cannot control neuronal activity independently at different locations. Here we describe equipment and provide a protocol for single-photon patterned optical stimulation of neurons using a digital micromirror device (DMD). This method can create arbitrary spatiotemporal light patterns with spatial and temporal resolutions in the micrometer and submillisecond range, respectively. Different options to integrate a DMD into a multiphoton microscope are presented and compared. We also describe an ex vivo preparation of the adult zebrafish head that greatly facilitates optogenetic and other experiments. After assembly, the initial alignment takes about one day and the zebrafish preparation takes

Details

ISSN :
17502799 and 17542189
Volume :
7
Database :
OpenAIRE
Journal :
Nature Protocols
Accession number :
edsair.doi.dedup.....bdcdd56ccc1918dc4abfff79f4798c08