Back to Search
Start Over
High-resolution optical control of spatiotemporal neuronal activity patterns in zebrafish using a digital micromirror device
- Source :
- Nature Protocols. 7:1410-1425
- Publication Year :
- 2012
- Publisher :
- Springer Science and Business Media LLC, 2012.
-
Abstract
- Optogenetic approaches allow the manipulation of neuronal activity patterns in space and time by light, particularly in small animals such as zebrafish. However, most techniques cannot control neuronal activity independently at different locations. Here we describe equipment and provide a protocol for single-photon patterned optical stimulation of neurons using a digital micromirror device (DMD). This method can create arbitrary spatiotemporal light patterns with spatial and temporal resolutions in the micrometer and submillisecond range, respectively. Different options to integrate a DMD into a multiphoton microscope are presented and compared. We also describe an ex vivo preparation of the adult zebrafish head that greatly facilitates optogenetic and other experiments. After assembly, the initial alignment takes about one day and the zebrafish preparation takes
- Subjects :
- Optics and Photonics
Microscope
Light
Photic Stimulation
Computer science
High resolution
Optogenetics
General Biochemistry, Genetics and Molecular Biology
law.invention
Digital micromirror device
Neurobiology
law
Animals
Premovement neuronal activity
Zebrafish
Neurons
Photons
biology
fungi
Optical Devices
Anatomy
biology.organism_classification
Olfactory bulb
Neuroscience
Subjects
Details
- ISSN :
- 17502799 and 17542189
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- Nature Protocols
- Accession number :
- edsair.doi.dedup.....bdcdd56ccc1918dc4abfff79f4798c08