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Transcriptional regulation of the bovine FGFR1 gene facilitates myoblast proliferation under hypomethylation of the promoter
- Source :
- Journal of Cellular Physiology. 235:8667-8678
- Publication Year :
- 2020
- Publisher :
- Wiley, 2020.
-
Abstract
- DNA methylation, which can affect the expression level of genes, is one of the most vital epigenetic modifications in mammals. Fibroblast growth factor receptor 1 (FGFR1) plays an important role in muscle development; however, DNA methylation of the FGFR1 promoter has not been studied to date in cattle. Our study focused on methylation of the FGFR1 promoter and its effect on bovine myoblast proliferation and differentiation. We identified the FGFR1 core promoter by using luciferase reporter assays; we then studied FGFR1 expression by reverse transcription quantitative polymerase chain reaction, and the methylation pattern in the FGFR1 core promoter by bisulfite sequencing polymerase chain reaction in bovine muscle tissue at three different developmental stages. We used RNAi strategy to investigate the function of FGFR1 in myoblast proliferation and differentiation. Results showed that the FGFR1 core promoters were located at the R2 (-509 to ~-202 bp) and R4 (-1295 to ~-794 bp) regions upstream of the FGFR1 gene. FGFR1 expression level was negatively associated with the degree of methylation of the FGFR1 core promoter during the developmental process. In addition, we found that FGFR1 can promote myoblast proliferation, but had no effect on myoblast differentiation. In conclusion, our results suggest that FGFR1 can promote myoblast proliferation and its transcription can be regulated by the methylation level of the core promoter. Our findings provide a mechanistic basis for the improvement of animal breeding.
- Subjects :
- 0301 basic medicine
Myoblast proliferation
Physiology
Muscle Fibers, Skeletal
Clinical Biochemistry
Bisulfite sequencing
Biology
Muscle Development
Myoblasts
03 medical and health sciences
0302 clinical medicine
Transcription (biology)
Humans
Receptor, Fibroblast Growth Factor, Type 1
Epigenetics
Muscle, Skeletal
Cell Proliferation
Binding Sites
Gene Expression Regulation, Developmental
Cell Differentiation
Promoter
Cell Biology
Methylation
DNA Methylation
Cell biology
stomatognathic diseases
030104 developmental biology
Real-time polymerase chain reaction
030220 oncology & carcinogenesis
DNA methylation
Subjects
Details
- ISSN :
- 10974652 and 00219541
- Volume :
- 235
- Database :
- OpenAIRE
- Journal :
- Journal of Cellular Physiology
- Accession number :
- edsair.doi.dedup.....bdc07c99b154d6182a22f1df140174fe