Back to Search
Start Over
G-Quadruplex-Based Fluorescent Turn-On Ligands and Aptamers: From Development to Applications
- Source :
- Molecules, Molecules, Vol 24, Iss 13, p 2416 (2019)
- Publication Year :
- 2019
- Publisher :
- MDPI AG, 2019.
-
Abstract
- Guanine (G)-quadruplexes (G4s) are unique nucleic acid structures that are formed by stacked G-tetrads in G-rich DNA or RNA sequences. G4s have been reported to play significant roles in various cellular events in both macro- and micro-organisms. The identification and characterization of G4s can help to understand their different biological roles and potential applications in diagnosis and therapy. In addition to biophysical and biochemical methods to interrogate G4 formation, G4 fluorescent turn-on ligands can be used to target and visualize G4 formation both in vitro and in cells. Here, we review several representative classes of G4 fluorescent turn-on ligands in terms of their interaction mechanism and application perspectives. Interestingly, G4 structures are commonly identified in DNA and RNA aptamers against targets that include proteins and small molecules, which can be utilized as G4 tools for diverse applications. We therefore also summarize the recent development of G4-containing aptamers and highlight their applications in biosensing, bioimaging, and therapy. Moreover, we discuss the current challenges and future perspectives of G4 fluorescent turn-on ligands and G4-containing aptamers.
- Subjects :
- Guanine
Aptamer
aptamers
Pharmaceutical Science
Computational biology
Review
turn-on ligands
010402 general chemistry
G-quadruplex
Ligands
01 natural sciences
Analytical Chemistry
lcsh:QD241-441
03 medical and health sciences
chemistry.chemical_compound
lcsh:Organic chemistry
Drug Discovery
Physical and Theoretical Chemistry
030304 developmental biology
0303 health sciences
Organic Chemistry
RNA
Aptamers, Nucleotide
Small molecule
0104 chemical sciences
G-Quadruplexes
nucleic acids
chemistry
Chemistry (miscellaneous)
Nucleic acid
Molecular Medicine
fluorescence
microbes
Biosensor
DNA
Subjects
Details
- ISSN :
- 14203049
- Volume :
- 24
- Issue :
- 13
- Database :
- OpenAIRE
- Journal :
- Molecules
- Accession number :
- edsair.doi.dedup.....bdaabccd29895fab1352fddddecfd1dc
- Full Text :
- https://doi.org/10.3390/molecules24132416