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The neuropeptides of Asian freshwater clam (Corbicula fluminea) as new molecular biomarker basing on the responses of organophosphate chemicals exposure
- Source :
- Ecotoxicology and environmental safety. 160
- Publication Year :
- 2018
-
Abstract
- In the present study, to discover new biomarker of Asian freshwater clam (Corbicula fluminea) to assess impact of environmental pollutions, cholecystokinin (CCK), conopressin, and Neuropeptide FF (FFamide) in C. fluminea were selected as potent biomarkers. Therefore, their full-length cDNAs were cloned and characterized to investigate the molecular characteristics and expression patterns of neuropeptides in C. fluminea. According to the sequence analysis, CCK, conopressin, and FFamide encoded proteins of 173, 152, and 90 amino acids, respectively. Moreover, the multiple sequence alignment revealed that the bioactive regions of these neuropeptides were well conserved among different invertebrates. In addition, under basal conditions, CCK, conopressin and FFamide mRNA were mainly expressed in the visceral mass, whereas the FFamide mRNA was rarely detected in the foot and mantle. Exposure to 20 and 200 μg/L Tris (2-butoxyethyl) phosphate (TBOEP) and tri-butyl-phosphate (TBP) exposure significantly up-regulated the expression of the CCK and FFamide mRNAs in the visceral mass (p 0.05), whereas no significant changes in conopressin mRNA levels were observed in response to any treatment. Therefore, CCK and FFamide of C. fluminea neuropeptides are feasible new biomarkers for screening and assessing responses to organophosphate chemicals.
- Subjects :
- 0301 basic medicine
Sequence analysis
Health, Toxicology and Mutagenesis
Neuropeptide
03 medical and health sciences
chemistry.chemical_compound
Animals
Neuropeptide FF
RNA, Messenger
Corbicula fluminea
Mantle (mollusc)
Corbicula
Cholecystokinin
chemistry.chemical_classification
biology
Organophosphate
Neuropeptides
Public Health, Environmental and Occupational Health
General Medicine
biology.organism_classification
Pollution
Organophosphates
Amino acid
Up-Regulation
030104 developmental biology
Biochemistry
chemistry
Biomarkers
Water Pollutants, Chemical
Subjects
Details
- ISSN :
- 10902414
- Volume :
- 160
- Database :
- OpenAIRE
- Journal :
- Ecotoxicology and environmental safety
- Accession number :
- edsair.doi.dedup.....bd8994fe779905f4ed3b996a7f15604e