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Pias3 is necessary for dorso-ventral patterning and visual response of retinal cones but is not required for rod photoreceptor differentiation
- Source :
- Biology Open, Vol 6, Iss 6, Pp 881-890 (2017), Biology Open, Biology Open, Royal Society, 2017, 6 (6), pp.881-890. ⟨10.1242/bio.024679⟩
- Publication Year :
- 2017
- Publisher :
- The Company of Biologists, 2017.
-
Abstract
- Protein inhibitor of activated Stat 3 (Pias3) is implicated in guiding specification of rod and cone photoreceptors through post-translational modification of key retinal transcription factors. To investigate its role during retinal development, we deleted exon 2-5 of the mouse Pias3 gene, which resulted in complete loss of the Pias3 protein. Pias3−/− mice did not show any overt phenotype, and retinal lamination appeared normal even at 18 months. We detected reduced photopic b-wave amplitude by electroretinography following green light stimulation of postnatal day (P)21 Pias3−/− retina, suggesting a compromised visual response of medium wavelength (M) cones. No change was evident in response of short wavelength (S) cones or rod photoreceptors until 7 months. Increased S-opsin expression in the M-cone dominant dorsal retina suggested altered distribution of cone photoreceptors. Transcriptome profiling of P21 and 18-month-old Pias3−/− retina revealed aberrant expression of a subset of photoreceptor genes. Our studies demonstrate functional redundancy in SUMOylation-associated transcriptional control mechanisms and identify a specific, though limited, role of Pias3 in modulating spatial patterning and optimal function of cone photoreceptor subtypes in the mouse retina.<br />Summary: Loss of Pias3 in mice results in altered dorso-ventral patterning of retinal cone photoreceptors by modulating the expression of a subset of genes, but does not affect rod development.
- Subjects :
- 0301 basic medicine
genetic structures
Vision
QH301-705.5
[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
Science
Stimulation
Biology
General Biochemistry, Genetics and Molecular Biology
Retina development
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
medicine
Mouse knockout
Protein inhibitor of activated STAT
Biology (General)
Genetics
Regulation of gene expression
Retina
medicine.diagnostic_test
[SDV.NEU.PC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Psychology and behavior
[SDV.NEU.SC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences
Retinal
eye diseases
SUMOylation
Cell biology
Gene regulation
030104 developmental biology
medicine.anatomical_structure
chemistry
Cell type specification
sense organs
General Agricultural and Biological Sciences
030217 neurology & neurosurgery
Electroretinography
Visual phototransduction
Photopic vision
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 20466390
- Volume :
- 6
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Biology Open
- Accession number :
- edsair.doi.dedup.....bd7d78f32005dbdabae56f131fee26a5