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Metabolic Potential of the Gut Microbiome Is Significantly Impacted by Conditioning Regimen in Allogeneic Hematopoietic Stem Cell Transplantation Recipients

Authors :
Mette Jørgensen
Jens C. Nørgaard
Emma E. Ilett
Ramtin Z. Marandi
Marc Noguera-Julian
Roger Paredes
Daniel D. Murray
Jens Lundgren
Cameron Ross MacPherson
Henrik Sengeløv
Source :
International Journal of Molecular Sciences, r-IGTP. Repositorio Institucional de Producción Científica del Instituto de Investigación Germans Trias i Pujol, instname, Jørgensen, M, Nørgaard, J C, Ilett, E E, Marandi, R Z, Noguera-Julian, M, Paredes, R, Murray, D D, Lundgren, J, MacPherson, C R & Sengeløv, H 2022, ' Metabolic Potential of the Gut Microbiome Is Significantly Impacted by Conditioning Regimen in Allogeneic Hematopoietic Stem Cell Transplantation Recipients ', International Journal of Molecular Sciences, vol. 23, no. 19, 11115 . https://doi.org/10.3390/ijms231911115, International Journal of Molecular Sciences; Volume 23; Issue 19; Pages: 11115
Publication Year :
2022
Publisher :
Multidisciplinary Digital Publishing Institute (MDPI), 2022.

Abstract

Allogeneic hematopoietic stem cell transplantation (aHSCT) is a putative curative treatment for malignant hematologic disorders. During transplantation, the immune system is suppressed/eradicated through a conditioning regimen (non-myeloablative or myeloablative) and replaced with a donor immune system. In our previous study, we showed changes in gut taxonomic profiles and a decrease in bacterial diversity post-transplant. In this study, we expand the cohort with 114 patients and focus on the impact of the conditioning regimens on taxonomic features and the metabolic functions of the gut bacteria. This is, to our knowledge, the first study to examine the metabolic potential of the gut microbiome in this patient group. Adult aHSCT recipients with shotgun sequenced stool samples collected day −30 to +28 relative to aHSCT were included. One sample was selected per patient per period: pre-aHSCT (day −30–0) and post-aHSCT (day 1–28). In total, 254 patients and 365 samples were included. Species richness, alpha diversity, gene richness and metabolic richness were all lower post-aHSCT than pre-aHSCT and the decline was more pronounced for the myeloablative group. The myeloablative group showed a decline in 36 genera and an increase in 15 genera. For the non-myeloablative group, 30 genera decreased and 16 increased with lower fold changes than observed in the myeloablative group. For the myeloablative group, 32 bacterial metabolic functions decreased, and one function increased. For the non-myeloablative group, three functions decreased, and two functions increased. Hence, the changes in taxonomy post-aHSCT caused a profound decline in bacterial metabolic functions especially in the myeloablative group, thus providing new evidence for associations of myeloablative conditioning and gut dysbiosis from a functional perspective.

Details

ISSN :
14220067
Database :
OpenAIRE
Journal :
International Journal of Molecular Sciences, r-IGTP. Repositorio Institucional de Producción Científica del Instituto de Investigación Germans Trias i Pujol, instname, Jørgensen, M, Nørgaard, J C, Ilett, E E, Marandi, R Z, Noguera-Julian, M, Paredes, R, Murray, D D, Lundgren, J, MacPherson, C R & Sengeløv, H 2022, ' Metabolic Potential of the Gut Microbiome Is Significantly Impacted by Conditioning Regimen in Allogeneic Hematopoietic Stem Cell Transplantation Recipients ', International Journal of Molecular Sciences, vol. 23, no. 19, 11115 . https://doi.org/10.3390/ijms231911115, International Journal of Molecular Sciences; Volume 23; Issue 19; Pages: 11115
Accession number :
edsair.doi.dedup.....bd77cc7d669793687f589a007a7432df
Full Text :
https://doi.org/10.3390/ijms231911115