Back to Search
Start Over
Whole-cell biocatalysts for stereoselective C-H amination reactions
- Source :
- Angewandte Chemie, International Edition, 55(4), 1511-1513. John Wiley and Sons Ltd
- Publication Year :
- 2016
-
Abstract
- Enantiomerically pure chiral amines are ubiquitous chemical building blocks in bioactive pharmaceutical products and their synthesis from simple starting materials is of great interest. One of the most attractive strategies is the stereoselective installation of a chiral amine through C H amination, which is a challenging chemical transformation. Herein we report the application of a multienzyme cascade, generated in a single bacterial whole-cell system, which is able to catalyze stereoselective benzylic aminations with ee values of 97.5 %. The cascade uses four heterologously expressed recombinant enzymes with cofactors provided by the host cell and isopropyl amine added as the amine donor. The cascade presents the first example of the successful de novo design of a single whole-cell biocatalyst for formal stereoselective C H amination.
- Subjects :
- Chemical transformation
Chromatography, Gas
010402 general chemistry
01 natural sciences
Catalysis
Cofactor
Escherichia coli
Organic chemistry
Biotransformation
Amination
chemistry.chemical_classification
biology
010405 organic chemistry
organic chemicals
Stereoisomerism
General Chemistry
Combinatorial chemistry
Carbon
0104 chemical sciences
Enzyme
chemistry
Biocatalysis
biology.protein
Amine gas treating
Stereoselectivity
Isopropyl
Hydrogen
Subjects
Details
- Language :
- English
- ISSN :
- 14337851
- Database :
- OpenAIRE
- Journal :
- Angewandte Chemie, International Edition, 55(4), 1511-1513. John Wiley and Sons Ltd
- Accession number :
- edsair.doi.dedup.....bd6ff1ce42ec1d7569b32efa02df1498