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The role of ERK and Smad2 signal pathways in the alternatively activated macrophages induced by TGF-β1 and high-ambient glucose
- Source :
- Journal of receptor and signal transduction research. 38(1)
- Publication Year :
- 2017
-
Abstract
- Macrophages can be alternatively activated by TGF-β1 and high-ambient glucose, in which the role of Smad2 and the crosstalk between ERK and Smad2 pathways are not fully understood. The activation of ERK and Smad2 pathways and the expression of arginase-1 were detected by Western blot. The role of Smad2 and the relationship between ERK and Smad2 pathways were investigated by using biochemical inhibitors. The protein of arginase-1 was significantly overexpressed in RAW264.7 cells stimulated by TGF-β1 and high-ambient glucose, which can be partially blocked by not only U0126 (ERK inhibitor) but also SB431542 (Smad2 inhibitor). Furthermore, simply inhibiting one pathway had no effect on the other pathway. In conclusion, both ERK and Smad2 signal pathways are involved in the activation of macrophages induced by TGF-β1 and high-ambient glucose, while there is no crosstalk shown in the process.
- Subjects :
- 0301 basic medicine
MAPK/ERK pathway
animal structures
MAP Kinase Signaling System
Dioxoles
Smad2 Protein
Biochemistry
Transforming Growth Factor beta1
03 medical and health sciences
Mice
stomatognathic system
Western blot
Nitriles
medicine
Butadienes
Animals
Phosphorylation
Molecular Biology
RAW 264.7 Cells
Signal Pathways
medicine.diagnostic_test
Chemistry
Macrophages
Cell Biology
Cell biology
Crosstalk (biology)
030104 developmental biology
Glucose
Benzamides
biological phenomena, cell phenomena, and immunity
Transforming growth factor
Subjects
Details
- ISSN :
- 15324281
- Volume :
- 38
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Journal of receptor and signal transduction research
- Accession number :
- edsair.doi.dedup.....bd67eef9ce81bf2ecdd5680b6b8cf120