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Members of the aquaporin family in the developing pea seed coat include representatives of the PIP, TIP, and NIP subfamilies
- Source :
- Plant molecular biology, 53(5), 655. Springer Verlag Gmbh (formerly Kluwer Academic Publishers), Scopus-Elsevier, Plant Molecular Biology, 53(5), 655. Springer Netherlands
- Publication Year :
- 2003
- Publisher :
- Springer Science and Business Media LLC, 2003.
-
Abstract
- Water and nutrients required by developing seeds are mainly supplied by the phloem and have to be released from a maternal parenchyma tissue before being utilized by the filial tissues of embryo and endosperm. To identify aquaporins that could be involved in this process four full-length cDNAs were cloned and sequenced from a cDNA library of developing seed coats of pea (Pisum sativum L.). The cDNA of PsPIP1-1 appeared to be identical to that of clone 7a/TRG-31, a turgor-responsive gene cloned previously from pea roots. PsPIP1-1, PsPIP2-1, and PsTIP1-1, or their possible close homologues, were also expressed in cotyledons of developing and germinating seeds, and in roots and shoots of seedlings, but transcripts of PsNIP-1 were only detected in the seed coat. In mature dry seeds, high hybridization signals were observed with the probe for PsPIP1-1, but transcripts of PsPIP2-1, PsTIP1-1, and PsNIP-1 were not detected. Functional characterization after heterologous expression in Xenopus oocytes showed that PsPIP2-1 and PsTIP1-1 are aquaporins whereas PsNIP-1 is an aquaglyceroporin. PsNIP-1, like several other NIPs, contains a tryptophan residue corresponding with Trp-48 in GlpF (the glycerol facilitator of Escherichia coli) that borders the selectivity filter in the permeation channel. It is suggested that PsPIP1-1 and/or its possible close homologues could play a role in water absorption during seed imbibition, and that PsPIP2-1, possibly together with PsPIP1-1, could be involved in the release of phloem water from the seed coat symplast, which is intimately connected with the release of nutrients for the embryo. Abbreviations: MIPs, major intrinsic proteins; NIPs, nodulin 26-like intrinsic proteins; PIPs, plasma membrane intrinsic proteins; SIPs, small, basic intrinsic proteins; TIPs, tonoplast intrinsic proteins
- Subjects :
- Osmosis
DNA, Complementary
Microinjections
Molecular Sequence Data
Aquaporin
Plant Science
Aquaporins
RNA, Complementary
Pisum
Endosperm
Xenopus laevis
Biologie/Milieukunde (BIOL)
Gene Expression Regulation, Plant
Complementary DNA
Taverne
Genetics
Animals
Amino Acid Sequence
RNA, Messenger
Cloning, Molecular
Plant biology (Botany)
Plant Proteins
Sequence Homology, Amino Acid
biology
cDNA library
Peas
Gene Expression Regulation, Developmental
Water
food and beverages
Symplast
Sequence Analysis, DNA
General Medicine
Blotting, Northern
biology.organism_classification
Life sciences
Biochemistry
Multigene Family
Seeds
Oocytes
Phloem
Heterologous expression
Sequence Alignment
Biologie
Agronomy and Crop Science
Subjects
Details
- ISSN :
- 01674412
- Volume :
- 53
- Database :
- OpenAIRE
- Journal :
- Plant Molecular Biology
- Accession number :
- edsair.doi.dedup.....bd39b1650fb6c66ef882d00491f27e62