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Intra-articular injection of magnesium chloride attenuates osteoarthritis progression in rats
- Source :
- Osteoarthritis and cartilage. 27(12)
- Publication Year :
- 2019
-
Abstract
- Summary Objective To explore the effects of Mg2+ on the expression of osteoarthritic markers in human cartilage and synovium tissue explants. To investigate the therapeutic effect of intra-articular injection of Mg2+ in an established rat OA (Osteoarthritis) model of anterior cruciate ligament transection with partial medial meniscectomy (ACLT + PMM). Design Human cartilage and synovium explants were collected from total knee replacement surgeries and incubated with MgCl2 (20 mmol/L) in vitro. A rat OA model was established by ACLT + PMM surgery in 450–500 g male Sprague Dawley (SD) rats. To select the optimal dose, intra-articular injections of MgCl2 (0.05, 0.5, 5 mol/L) were performed at 4 weeks after the surgery every 3 days for 2 weeks. The effect of optimized MgCl2 was further determined by histology, immunohistochemistry, and quantitative real-time polymerase chain reaction. Results The expressions of osteoarthritic markers in human cartilage and synovium explants were inhibited by Mg2+ in vitro. Immunohistochemical analysis further suggested the inhibitory effects of Mg2+ on the expression of MMP-13 and IL-6 in the human tissue explants. Cartilage degeneration and synovitis in ACLT + PMM rats were significantly improved by intra-articular injections of Mg2+ (0.5 mol/L). Immunohistochemical analysis also showed the regulatory effects of Mg2+ on osteoarthritic markers in both cartilage and synovium in rats, consistent with in vitro results. Conclusion Intra-articular injections of Mg2+ at 0.5 mol/L attenuate the progression of OA in the ACLT + PMM rat model. Such effect was at least in part explained by the promotion of cartilage matrix synthesis and the suppression of synovial inflammation.
- Subjects :
- 0301 basic medicine
Cartilage, Articular
Male
Pathology
Interleukin-1beta
Osteoarthritis
Injections, Intra-Articular
0302 clinical medicine
ADAMTS Proteins
Orthopedics and Sports Medicine
Aggrecans
Anterior Cruciate Ligament
Arthroplasty, Replacement, Knee
Synovitis
Synovial Membrane
Osteoarthritis, Knee
Immunohistochemistry
medicine.anatomical_structure
Matrix Metalloproteinase 9
Female
medicine.symptom
medicine.medical_specialty
Anterior cruciate ligament
Biomedical Engineering
Magnesium Chloride
Inflammation
In Vitro Techniques
Real-Time Polymerase Chain Reaction
03 medical and health sciences
Rheumatology
Matrix Metalloproteinase 13
medicine
Animals
Humans
RNA, Messenger
Collagen Type II
Aged
Meniscectomy
030203 arthritis & rheumatology
business.industry
Interleukin-6
Cartilage
Histology
medicine.disease
Rats
Disease Models, Animal
030104 developmental biology
business
Explant culture
Subjects
Details
- ISSN :
- 15229653
- Volume :
- 27
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- Osteoarthritis and cartilage
- Accession number :
- edsair.doi.dedup.....bd2cf4eb140844c27002e9b7ff59d292