Back to Search
Start Over
Analysis of the molecular and biological variability of Zucchini yellow mosaic virus isolates from Iran and Iraq
- Source :
- Gene. 788
- Publication Year :
- 2021
-
Abstract
- During the growing season of 2018, several field-grown cucurbit plants in different parts of Iraq and Iran were surveyed for the presence of zucchini yellow mosaic virus (ZYMV), using two degenerate primer pairs (CIF/Rev and NIb2F/3R) targeting the two separated partial regions of the potyvirus genome (CI and NIb respectively). 7 out of 20 samples were confirmed to be infected with ZYMV. Phylogenetic analyses based on the CI gene grouped all Iranian and two Iraqi (ZYMV1 and ZYMV2) isolates together with isolates from the Middle East in the subgroup (AI), whereas the other Iraqi (ZYMV3 and ZYMV4) isolates were clustered in the subgroup (DI), which was only consisted of American isolates. The highest and lowest identity between the studied isolates and the GenBank isolates showed that the two genes (CI, NIb) of each isolate particularly the Iraqi isolates were more similar to a specific and geographically scattered mosaic of worldwide isolates, suggestive of mixed infection might have occurred between different worldwide isolates in Iraq. Furthermore, the first complete nucleotide sequence of an Iraqi ZYMV (ZYMV-Iq) isolate was done, using the Illumina sequencing technique. The complete nucleotide sequence of ZYMV-Iq isolate was 9650 nt, excluding the 3′poly (A) tail. ZYMV-Iq isolate shared the highest nt identity of 98.8% with an American (KC665630) isolate. Phylogenetic analysis based on the full genome sequence placed ZYMV-Iq in subgroup A of group I alongside 18 isolates from the US and two isolates from Australia. In addition, recombination analysis detected lone significant recombination between ZYMV-Iq and South Korean (AY279000) isolate. Moreover, the results showed that symptom intensity was varied across experimental host plants.
- Subjects :
- 0301 basic medicine
Veterinary medicine
Potyvirus
Genome, Viral
Iran
03 medical and health sciences
0302 clinical medicine
Cucurbita
Genome Size
Genetics
Illumina dye sequencing
Phylogeny
Whole genome sequencing
Recombination, Genetic
Genetic diversity
Zucchini yellow mosaic virus
biology
Phylogenetic tree
Whole Genome Sequencing
Sequence Analysis, RNA
Nucleic acid sequence
Australia
High-Throughput Nucleotide Sequencing
General Medicine
biology.organism_classification
United States
Phylogeography
030104 developmental biology
030220 oncology & carcinogenesis
GenBank
Iraq
Subjects
Details
- ISSN :
- 18790038
- Volume :
- 788
- Database :
- OpenAIRE
- Journal :
- Gene
- Accession number :
- edsair.doi.dedup.....bd1f0d39d5d36a49d45d0794d607ea42