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Inhibition of acid secretory response and induction of ornithine decarboxylase and its mRNA by TGF alpha and EGF in isolated rat gastric glands
- Source :
- Regulatory peptides. 56(1)
- Publication Year :
- 1995
-
Abstract
- TGF alpha shows structural and functional homology to EGF, but TGF alpha's mitogenic potency is greater. Our previous study showed that EGF may inhibit parietal cell secretory response through the induction of ornithine decarboxylase (ODC). The aim of this study was to determine parietal cell acid production in vitro in response to stimulation by TGF alpha and EGF and to compare the effect of these two growth factors on ODC activity and ODC mRNA in isolated rat gastric glands. 45 min treatment with TGF alpha and EGF had no effect on basal acid production but did inhibit histamine-stimulated acid production in a dose dependent manner. The two growth factors did not inhibit histamine-stimulated aminopyrine (AP) uptake from incubation medium with concentration of KCl increased from 5 to 70 mM. In the presence of specific ODC inhibitor, alpha-difluoromethylornithine (DFMO), both EGF and TGF alpha failed to inhibit histamine-stimulated AP accumulation. Polyamine spermine also inhibited AP accumulation but this inhibitory effect was not affected by DFMO. After 1 h treatment with TGF alpha and EGF, ODC activities increased to an average 283 +/- 78% and 227 +/- 64% above the basal activity, respectively. 30 min treatment of gastric glands with TGF alpha and EGF resulted in, respectively, 2.9 +/- 0.4- and 2.7 +/- 0.5-fold increases of ODC mRNA level, as assessed by RT-PCR.(ABSTRACT TRUNCATED AT 250 WORDS)
- Subjects :
- medicine.medical_specialty
TGF alpha
Eflornithine
Physiology
Clinical Biochemistry
Molecular Sequence Data
Spermine
Biology
In Vitro Techniques
Ornithine Decarboxylase
Biochemistry
Polymerase Chain Reaction
Ornithine decarboxylase
Gastric Acid
Cellular and Molecular Neuroscience
chemistry.chemical_compound
Endocrinology
Epidermal growth factor
Internal medicine
Gastric glands
medicine
Gastric mucosa
Animals
RNA, Messenger
Rats, Wistar
Aminopyrine
Parietal cell
DNA Primers
Base Sequence
Epidermal Growth Factor
Transforming Growth Factor alpha
Rats
medicine.anatomical_structure
chemistry
Gastric Mucosa
Enzyme Induction
Gastric acid
Histamine
Subjects
Details
- ISSN :
- 01670115
- Volume :
- 56
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Regulatory peptides
- Accession number :
- edsair.doi.dedup.....bd1d7d076b4d323c565fec2742d47a9a