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Structural organization of the polysomes adjacent to mammalian processing bodies (P-bodies)
Structural organization of the polysomes adjacent to mammalian processing bodies (P-bodies)
- Source :
- RNA Biology, RNA Biology, Taylor & Francis, 2013, 10 (2), pp.314-20. ⟨10.4161/rna.23342⟩, RNA Biology, 2013, 10 (2), pp.314-20. ⟨10.4161/rna.23342⟩
- Publication Year :
- 2013
- Publisher :
- Informa UK Limited, 2013.
-
Abstract
- International audience; A finely tuned balance of translation, storage and decay of mRNAs (mRNAs) is important for the regulation of gene expression. In eukaryotic cells, this takes place in dynamic cytoplasmic RNA-protein granules termed Processing bodies (P-bodies). In this study, by using immunoelectron tomography, 3D modeling and template matching, we analyze the size and the organization of the polysomes in the vicinity of human P-bodies. Our results show the presence of several polysomes that are compatible with a translational activity around P-bodies. Therefore, movement of mRNAs between polysomes and P-bodies can take place when the two compartments are in close contact. The presence of initiation factors in the proximity of P-bodies also suggests that translation of mRNAs can resume at the periphery of these granules.
- Subjects :
- Electron Microscope Tomography
RNA Stability
mRNA
Eukaryotic Initiation Factor-4E
electron tomography
[SDV.GEN] Life Sciences [q-bio]/Genetics
Biology
Cytoplasmic Granules
Ribosome
RNA Transport
ribosomes
03 medical and health sciences
Polysome
P-bodies
Protein biosynthesis
Humans
Initiation factor
RNA, Messenger
Molecular Biology
030304 developmental biology
polysomes
Regulation of gene expression
[SDV.GEN]Life Sciences [q-bio]/Genetics
0303 health sciences
030302 biochemistry & molecular biology
Translation (biology)
Cell Biology
initiation
Cell biology
Polyribosomes
Protein Biosynthesis
Eukaryotic Initiation Factor-4G
HeLa Cells
Research Paper
Subjects
Details
- ISSN :
- 15558584 and 15476286
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- RNA Biology
- Accession number :
- edsair.doi.dedup.....bd14929cba21c1091a58e88b93551716
- Full Text :
- https://doi.org/10.4161/rna.23342