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Structural organization of the polysomes adjacent to mammalian processing bodies (P-bodies)

Structural organization of the polysomes adjacent to mammalian processing bodies (P-bodies)

Authors :
Reynald Gillet
Emmanuel Giudice
Annie Cavalier
Anne-Elisabeth Molza
Daniel Thomas
Nicolas Cougot
Institut de Génétique et Développement de Rennes (IGDR)
Université de Rennes 1 (UR1)
Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )
Touya, Véronique
Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )
Source :
RNA Biology, RNA Biology, Taylor & Francis, 2013, 10 (2), pp.314-20. ⟨10.4161/rna.23342⟩, RNA Biology, 2013, 10 (2), pp.314-20. ⟨10.4161/rna.23342⟩
Publication Year :
2013
Publisher :
Informa UK Limited, 2013.

Abstract

International audience; A finely tuned balance of translation, storage and decay of mRNAs (mRNAs) is important for the regulation of gene expression. In eukaryotic cells, this takes place in dynamic cytoplasmic RNA-protein granules termed Processing bodies (P-bodies). In this study, by using immunoelectron tomography, 3D modeling and template matching, we analyze the size and the organization of the polysomes in the vicinity of human P-bodies. Our results show the presence of several polysomes that are compatible with a translational activity around P-bodies. Therefore, movement of mRNAs between polysomes and P-bodies can take place when the two compartments are in close contact. The presence of initiation factors in the proximity of P-bodies also suggests that translation of mRNAs can resume at the periphery of these granules.

Details

ISSN :
15558584 and 15476286
Volume :
10
Database :
OpenAIRE
Journal :
RNA Biology
Accession number :
edsair.doi.dedup.....bd14929cba21c1091a58e88b93551716
Full Text :
https://doi.org/10.4161/rna.23342