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Isolation of human germ-line DNA suitable for recombinant DNA studies
- Source :
- Gene. 7(3-4)
- Publication Year :
- 1979
-
Abstract
- Dna from human sperm cells can be isolated with size and purity sufficient for use in recombinant DNA research. The DNA averages 100 000 base pairs (bp) in size (about 70.10(6) daltons) and is free of somatic cell, bacterial, and viral DNA. It can therefore be cloned under P2 + EK2 conditions as stipulated in the 1978 NIH Guidelines for recombinant DNA research.
- Subjects :
- Male
Base pair
DNA, Recombinant
Molecular cloning
Biology
law.invention
chemistry.chemical_compound
law
Genetics
Centrifugation, Density Gradient
Humans
Genomic library
Cloning, Molecular
Cloning
food and beverages
General Medicine
DNA
Containment of Biohazards
Molecular biology
DNA extraction
Spermatozoa
Molecular Weight
chemistry
Recombinant DNA
In vitro recombination
Subjects
Details
- ISSN :
- 03781119
- Volume :
- 7
- Issue :
- 3-4
- Database :
- OpenAIRE
- Journal :
- Gene
- Accession number :
- edsair.doi.dedup.....bd10a46fd7ce70f7c30f69879a193fdd