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Evidencing 98 secondary metabolites of Penicillium verrucosum using substrate isotopic labeling and high-resolution mass spectrometry
- Source :
- Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, Elsevier, 2017, in press, ⟨10.1016/j.jchromb.2017.03.011⟩, Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2017, in press, ⟨10.1016/j.jchromb.2017.03.011⟩, Journal of Chromatography. B (1071), 29-43. (2017)
- Publication Year :
- 2017
- Publisher :
- HAL CCSD, 2017.
-
Abstract
- Industrial applications of fungal compounds, coupled with the emergence of fungal threats to natural ecosystems and public health, have increased interest in filamentous fungi. Among all pathogenic fungi, Penicillium verrucosum is one of the most common mold-infecting stored cereals in temperate regions. However, it is estimated that 80% of fungal secondary metabolites remain unknown. To detect new P. verrucosum compounds, an untargeted metabolomic approach was applied to fungus grown on wheat grains labeled with stable isotopes: (i) natural grains (99% 12C); (ii) grains enriched with 97% of 13C; and (iii) grains enriched with 53% of 13C and 97% of 15N. Analyses performed by high-performance liquid chromatography coupled with high-resolution mass spectrometry (HPLC-HRMS) enabled the specific detection of fungal metabolites, and the unambiguous characterization of their chemical formulas. In this way, 98 secondary metabolites were detected and their chemical formulas were determined. Of these, only 18 identifications could be made based on databases, the literature and mass spectrometry fragmentation experiments, with the result that 80 were totally unknown. Molecular networks were generated to analyze these results, leading to the characterization by MSn experiments of a new fungisporin produced by P. verrucosum. More generally, this article provides precise mass spectrometric data about all these compounds for further studies of the Penicillium metabolome.
- Subjects :
- 0301 basic medicine
penicillium verrucosum
[SDV]Life Sciences [q-bio]
Clinical Biochemistry
mass spectrum analysis
toxicologie alimentaire
stable isotope labeling
Fungus
secondary metabolite
Secondary metabolite
Mass spectrometry
Biochemistry
Analytical Chemistry
Isotopic labeling
03 medical and health sciences
Metabolomics
spectrométrie de masse
medicine
Penicillium verrucosum
Metabolome
Chromatography, High Pressure Liquid
mass spectrometry
molecular networking
Chromatography
biology
Chemistry
public health
fungus
Penicillium
Cell Biology
General Medicine
biology.organism_classification
030104 developmental biology
métabolite secondaire
santé publique
Isotope Labeling
secondary plant products
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 15700232 and 1873376X
- Database :
- OpenAIRE
- Journal :
- Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, Elsevier, 2017, in press, ⟨10.1016/j.jchromb.2017.03.011⟩, Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2017, in press, ⟨10.1016/j.jchromb.2017.03.011⟩, Journal of Chromatography. B (1071), 29-43. (2017)
- Accession number :
- edsair.doi.dedup.....bcdc19cd74e76f8f01681c2f2fbc7fbb