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Surface-enhanced Raman scattering: a structure-specific detection method for capillary electrophoresis

Authors :
Christine D. Keating
Lin He
Michael J. Natan
Source :
Analytical chemistry. 72(21)
Publication Year :
2000

Abstract

A new approach to detecting capillary electrophoresis (CE) eluent components by interfacing CE with a surface-enhanced Raman scattering (SERS) system is described. In this approach, CE-based separation of a mixture of trans-1,2-bis(4-pyridyl)ethylene and N,N-dimethyl-4-nitrosoaniline has been detected by SERS in a postcolumn geometry. The retention time obtained from SERS corresponds well with that from conventional UV−visible detection. Meanwhile, CE eluants are identified by their characteristic vibrational spectra, demonstrating the validity of SERS as a structure-specific detection method for CE. In addition, the ability to monitor SERS intensity changes at molecule-specific frequencies makes selective detection of individual analytes possible, even when separation is incomplete. Finally, CE-SERS is evaluated for separation of amino acids (tyrosine and tryptophan) and environmental pollutants (chlorophenol mixtures).

Details

ISSN :
00032700
Volume :
72
Issue :
21
Database :
OpenAIRE
Journal :
Analytical chemistry
Accession number :
edsair.doi.dedup.....bcd8209dfc962cec37308f47ef4208ea