Back to Search Start Over

Development and evaluation of a calibrator material for nucleic acid-based assays for diagnosing aspergillosis

Authors :
Lyon, G.M.
Abdul-Ali, D.
Loeffler, J.
White, P.L.
Wickes, B.
Herrera, M.L.
Alexander, B.D.
Baden, L.R.
Clancy, C.
Denning, D.
Nguyen, M.H.
Sugrue, M.
Wheat, L.J.
Wingard, J.R.
Donnelly, J.P.
Barnes, R.
Patterson, T.F.
Caliendo, A.M.
Lyon, G.
Marty, F.
Bretagne, S.
Melchers, W.J.
White, P.
Klingspor, L.
Cuenca-Estrella, M.
Source :
Journal of Clinical Microbiology, 51, 7, pp. 2403-5, Journal of Clinical Microbiology, 51, 2403-5
Publication Year :
2013

Abstract

Twelve laboratories evaluated candidate material for an Aspergillus DNA calibrator. The DNA material was quantified using limiting-dilution analysis; the mean concentration was determined to be 1.73 × 10 10 units/ml. The calibrator can be used to standardize aspergillosis diagnostic assays which detect and/or quantify nucleic acid.

Details

ISSN :
00951137
Database :
OpenAIRE
Journal :
Journal of Clinical Microbiology, 51, 7, pp. 2403-5, Journal of Clinical Microbiology, 51, 2403-5
Accession number :
edsair.doi.dedup.....bcb73a2a926c0367d34d4b3e7057a38f