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Recovery and function of human fetal pancreas frozen to -196 C
- Source :
- Transplantation. 32(1)
- Publication Year :
- 1981
-
Abstract
- Human fetal pancreases were dissected into 1- to 2- mm3 fragments and frozen to -196 C using a modification of procedures previously used successfully for cryopreservation of the rat fetal pancreas. Freeze-recovered pancreatic pieces were able to incorporate radioactive amino acids into proteins. When placed into organ culture, nonfrozen control and freeze-recovered pancreas tissue secreted radioimmoassayable insulin in response to glucose plus theophylline, but not to glucose alone. Freeze-killed pancreatic pieces were totally inactive functionally. With respect to those parameters tested, freeze-recovered pancreas tissues were equivalent to nonfrozen tissue.
- Subjects :
- Cell Survival
medicine.medical_treatment
Organ culture
Cryopreservation
Andrology
Islets of Langerhans
Fetus
Freezing
Insulin Secretion
medicine
Humans
Insulin
Theophylline
Pancreas
chemistry.chemical_classification
Transplantation
Chemistry
Organ Preservation
Amino acid
medicine.anatomical_structure
Tissue Preservation
Function (biology)
medicine.drug
Subjects
Details
- ISSN :
- 00411337
- Volume :
- 32
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Transplantation
- Accession number :
- edsair.doi.dedup.....bc9b2992e01dc5af74781ab6ddd56e8c