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Quiescence of human muscle stem cells is favored by culture on natural biopolymeric films
- Source :
- Stem Cell Research & Therapy, Stem Cell Research and Therapy, Stem Cell Research and Therapy, BioMed Central, 2017, 8, pp.104. ⟨10.1186/s13287-017-0556-8⟩, Stem Cell Research and Therapy, 2017, 8, pp.104. ⟨10.1186/s13287-017-0556-8⟩, Stem Cell Research & Therapy, Vol 8, Iss 1, Pp 1-13 (2017)
- Publication Year :
- 2017
- Publisher :
- BioMed Central, 2017.
-
Abstract
- Background Satellite cells are quiescent resident muscle stem cells that present an important potential to regenerate damaged tissue. However, this potential is diminished once they are removed from their niche environment in vivo, prohibiting the long-term study and genetic investigation of these cells. This study therefore aimed to provide a novel biomaterial platform for the in-vitro culture of human satellite cells that maintains their stem-like quiescent state, an important step for cell therapeutic studies. Methods Human muscle satellite cells were isolated from two donors and cultured on soft biopolymeric films of controlled stiffness. Cell adhesive phenotype, maintenance of satellite cell quiescence and capacity for gene manipulation were investigated using FACS, western blotting, fluorescence microscopy and electron microscopy. Results About 85% of satellite cells cultured in vitro on soft biopolymer films for 3 days maintained expression of the quiescence marker Pax7, as compared with 60% on stiffer films and 50% on tissue culture plastic. The soft biopolymeric films allowed satellite cell culture for up to 6 days without renewing the media. These cells retained their stem-like properties, as evidenced by the expression of stem cell markers and reduced expression of differentiated markers. In addition, 95% of cells grown on these soft biopolymeric films were in the G0/G1 stage of the cell cycle, as opposed to those grown on plastic that became activated and began to proliferate and differentiate. Conclusions Our study identifies a new biomaterial made of a biopolymer thin film for the maintenance of the quiescence state of muscle satellite cells. These cells could be activated at any point simply by replating them onto a plastic culture dish. Furthermore, these cells could be genetically manipulated by viral transduction, showing that this biomaterial may be further used for therapeutic strategies. Electronic supplementary material The online version of this article (doi:10.1186/s13287-017-0556-8) contains supplementary material, which is available to authorized users.
- Subjects :
- 0301 basic medicine
Male
Satellite Cells, Skeletal Muscle
Cellular differentiation
Primary Cell Culture
Medicine (miscellaneous)
Culture platform
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
Biology
Quiescence
Stem cell marker
Biochemistry, Genetics and Molecular Biology (miscellaneous)
Biomimetism
lcsh:Biochemistry
03 medical and health sciences
Tissue culture
Biopolymers
Cell quiescence
Satellite cells
Humans
lcsh:QD415-436
[SDV.BC] Life Sciences [q-bio]/Cellular Biology
Cells, Cultured
Cell Proliferation
lcsh:R5-920
[SDV.MHEP] Life Sciences [q-bio]/Human health and pathology
Polymeric biomaterial
Layer by layer
Tissue Scaffolds
Cell growth
Research
Cell Differentiation
Cell Biology
Anatomy
Middle Aged
Cell biology
Culture Media
Adult Stem Cells
030104 developmental biology
Cell culture
Molecular Medicine
Stem cell
lcsh:Medicine (General)
[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Adult stem cell
Subjects
Details
- Language :
- English
- ISSN :
- 17576512
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- Stem Cell Research & Therapy
- Accession number :
- edsair.doi.dedup.....bc978b1bfc4e0a8b26c7df2e8b7a6867