Back to Search
Start Over
Solid-phase synthesis of pseudo-complementary peptide nucleic acids
- Source :
- Nature Protocols. 3:646-654
- Publication Year :
- 2008
- Publisher :
- Springer Science and Business Media LLC, 2008.
-
Abstract
- Pseudo-complementary peptide nucleic acid (pcPNA) is a DNA analog in which modified DNA bases 2,6-diaminopurine (D) and 2-thiouracil (U(s)) 'decorate' a poly[N-(2-aminoethyl)glycine] backbone, together with guanine (G) and cytosine (C). One of the most significant characteristics of pcPNA is its ability to effect double-duplex invasion of predetermined DNA sites inducing various changes in the biological and the physicochemical properties of the DNA. This protocol describes solid-phase synthesis of pcPNA. The monomers for G and C are commercially available, but the monomers for D and U(s) need to be synthesized (or can be ordered to custom synthesis companies). Otherwise, the procedure is the same as that employed for Boc-strategy synthesis of conventional PNA. This protocol, if the synthesis of D and U(s) monomers is not factored in, takes approximately 7 d to complete.
- Subjects :
- Peptide Nucleic Acids
chemistry.chemical_classification
Molecular Structure
Peptide nucleic acid
Guanine
Stereochemistry
Peptide
Thiouracil
General Biochemistry, Genetics and Molecular Biology
chemistry.chemical_compound
Solid-phase synthesis
chemistry
Biochemistry
Nucleic acid
A-DNA
2-Aminopurine
Cytosine
DNA
Subjects
Details
- ISSN :
- 17502799 and 17542189
- Volume :
- 3
- Database :
- OpenAIRE
- Journal :
- Nature Protocols
- Accession number :
- edsair.doi.dedup.....bc933689bffadc567d7a9921d5a9ffe1