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Pinocembrin Inhibits the Proliferation, Migration, Invasiveness, and Epithelial-Mesenchymal Transition of Colorectal Cancer Cells by Regulating LACTB
- Source :
- Cancer Biotherapy and Radiopharmaceuticals. 37:527-536
- Publication Year :
- 2022
- Publisher :
- Mary Ann Liebert Inc, 2022.
-
Abstract
- Background: Colorectal cancer (CRC) is a common malignancy of digestive tract. Pinocembrin (PINO) has been discovered to have proapoptotic effect on CRC. This study aimed to elucidate how other biological behaviors of CRC cells were affected under PINO treatment. Materials & Methods: The effect of PINO on HT29 and HCT116 cells were detected through treatment of different concentrations of PINO. The role of LACTB in PINO treatment was investigated by transfection of siRNA-LACTB. Cell counting kit-8 assay, wound healing assay, and Transwell assay were conducted to evaluate the proliferation, migration, and invasiveness of CRC cells, respectively. Western blot or quantitative reverse transcription-polymerase chain reaction was carried out to measure the expressions of LACTB, matrix metalloproteinase (MMP)-2, E-cadherin, and N-cadherin. Results: Gradient PINO inhibited the viability, migration, invasiveness, and expressions of MMP-2 and N-cadherin in CRC cells, while promoted E-cadherin and LACTB expressions. Silencing LACTB promoted the viability, migration, invasiveness, and expressions of MMP-2 and N-cadherin in CRC cells and inhibited E-cadherin expression. PINO counteracted the effect of silenced LACTB, and yet silencing LACTB partially abolished the effect of PINO on CRC cells. Conclusion: PINO inhibited the proliferation, migration, invasiveness, and epithelial-to-mesenchymal transition of CRC cells by regulating LACTB.
- Subjects :
- Cancer Research
Epithelial-Mesenchymal Transition
Colorectal cancer
Matrix metalloproteinase
beta-Lactamases
Mitochondrial Proteins
chemistry.chemical_compound
Western blot
Cell Movement
Cell Line, Tumor
medicine
Humans
Gene silencing
Neoplasm Invasiveness
Radiology, Nuclear Medicine and imaging
Epithelial–mesenchymal transition
neoplasms
Cell Proliferation
Pharmacology
Pinocembrin
Transition (genetics)
medicine.diagnostic_test
Chemistry
Membrane Proteins
General Medicine
Transfection
Cadherins
medicine.disease
digestive system diseases
Gene Expression Regulation, Neoplastic
Oncology
Flavanones
Cancer research
Matrix Metalloproteinase 2
Colorectal Neoplasms
Subjects
Details
- ISSN :
- 15578852 and 10849785
- Volume :
- 37
- Database :
- OpenAIRE
- Journal :
- Cancer Biotherapy and Radiopharmaceuticals
- Accession number :
- edsair.doi.dedup.....bc8a4e41d02a21aa9cc7d19150ea4dc8
- Full Text :
- https://doi.org/10.1089/cbr.2020.4052