Back to Search Start Over

Pinocembrin Inhibits the Proliferation, Migration, Invasiveness, and Epithelial-Mesenchymal Transition of Colorectal Cancer Cells by Regulating LACTB

Authors :
Haiyang Feng
Yong Liu
Yongbo Yang
Lai Jiang
Qinfei Zhou
Source :
Cancer Biotherapy and Radiopharmaceuticals. 37:527-536
Publication Year :
2022
Publisher :
Mary Ann Liebert Inc, 2022.

Abstract

Background: Colorectal cancer (CRC) is a common malignancy of digestive tract. Pinocembrin (PINO) has been discovered to have proapoptotic effect on CRC. This study aimed to elucidate how other biological behaviors of CRC cells were affected under PINO treatment. Materials & Methods: The effect of PINO on HT29 and HCT116 cells were detected through treatment of different concentrations of PINO. The role of LACTB in PINO treatment was investigated by transfection of siRNA-LACTB. Cell counting kit-8 assay, wound healing assay, and Transwell assay were conducted to evaluate the proliferation, migration, and invasiveness of CRC cells, respectively. Western blot or quantitative reverse transcription-polymerase chain reaction was carried out to measure the expressions of LACTB, matrix metalloproteinase (MMP)-2, E-cadherin, and N-cadherin. Results: Gradient PINO inhibited the viability, migration, invasiveness, and expressions of MMP-2 and N-cadherin in CRC cells, while promoted E-cadherin and LACTB expressions. Silencing LACTB promoted the viability, migration, invasiveness, and expressions of MMP-2 and N-cadherin in CRC cells and inhibited E-cadherin expression. PINO counteracted the effect of silenced LACTB, and yet silencing LACTB partially abolished the effect of PINO on CRC cells. Conclusion: PINO inhibited the proliferation, migration, invasiveness, and epithelial-to-mesenchymal transition of CRC cells by regulating LACTB.

Details

ISSN :
15578852 and 10849785
Volume :
37
Database :
OpenAIRE
Journal :
Cancer Biotherapy and Radiopharmaceuticals
Accession number :
edsair.doi.dedup.....bc8a4e41d02a21aa9cc7d19150ea4dc8
Full Text :
https://doi.org/10.1089/cbr.2020.4052