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Practical mechanical threshold estimation in rodents using von Frey hairs/Semmes–Weinstein monofilaments: Towards a rational method
- Source :
- Journal of Neuroscience Methods. 255:92-103
- Publication Year :
- 2015
- Publisher :
- Elsevier BV, 2015.
-
Abstract
- Here, we reconsider the status quo in testing mechanical sensitivity with von Frey's hairs. The aim is to improve paw withdrawal estimates by integrating current psychometric theory, and to maximise the clinical relevance and statistical power of mechanosensory models. A wealth of research into human tactile stimulus perception may be extended to the quantification of laboratory animal behaviour. We start by reviewing each step of the test, from its design and application through to data analysis. Filament range is assessed as a whole; possible test designs are compared; techniques of filament application to mice and rats are considered; curve fitting software is introduced; possibilities for data pooling and curve fitting are evaluated. A rational update of classical methods in line with recent advances in psychometrics and supported by open source software is expected to improve data homogeneity, and Reduce and Refine animal use in accord with the ‘3R’ principles.
- Subjects :
- Pain Threshold
Allodynia
Esthesiometer
Rodent behaviour
Semmes–Weinstein monofilaments
Threshold estimation
Von Frey hairs
Data Pooling
Psychometrics
Motor Activity
Machine learning
computer.software_genre
Statistical power
Mice
Software
Physical Stimulation
Rational method
Animals
Pain Measurement
Animal use
business.industry
General Neuroscience
Models, Theoretical
Rats
Von frey
Curve fitting
Artificial intelligence
business
Psychology
computer
Neuroscience
Subjects
Details
- ISSN :
- 01650270
- Volume :
- 255
- Database :
- OpenAIRE
- Journal :
- Journal of Neuroscience Methods
- Accession number :
- edsair.doi.dedup.....bc87654b2c54d969c7af852631839901