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Enhanced Cell Growth of Adipocyte-Derived Mesenchymal Stem Cells Using Chemically-Defined Serum-Free Media
- Source :
- International Journal of Molecular Sciences, Vol 18, Iss 8, p 1779 (2017), International Journal of Molecular Sciences; Volume 18; Issue 8; Pages: 1779, International Journal of Molecular Sciences
- Publication Year :
- 2017
- Publisher :
- MDPI AG, 2017.
-
Abstract
- The multipotency and anti-inflammatory effects of mesenchymal stem cells (MSCs) make them attractive for cell therapy in regenerative medicine. A large number of MSCs is required for efficient therapy owing to the low homing efficiency of MSCs to target sites. Furthermore, owing to limitations in obtaining sufficient amounts of MSCs, in vitro expansion of MSCs that preserves their differentiation and proliferative potential is essential. The animal factor included in culture media also limits clinical application. In this study, adipose-derived MSCs showed a significantly higher proliferation rate in STK2, a chemically-defined medium, than in DMEM/FBS. The expression of MSC surface markers was increased in the culture using STK2 compared to that using DMEM/FBS. Tri-lineage differentiation analyses showed that MSCs cultured in STK2 were superior to those cultured in DMEM/FBS. In addition, MSCs cultured in STK2 showed a reduced senescence rate, small and homogenous cell size, and were more genetically stable compared to those cultured in DMEM/FBS. Furthermore, secretome analysis showed that the expression of factors related to proliferation/migration, anti-inflammation, and differentiation were increased in STK2 culture medium compared to DMEM/FBS. Taken together, these results suggest that culture using STK2 medium offers many advantages through which it is possible to obtain safer, superior, and larger numbers of MSCs.
- Subjects :
- 0301 basic medicine
proliferation
Cell Culture Techniques
Biology
Regenerative medicine
Catalysis
Article
Inorganic Chemistry
Cell therapy
chemically-defined medium
lcsh:Chemistry
03 medical and health sciences
chemistry.chemical_compound
mesenchymal stem cell
multipotency
differentiation
secretome
Adipocyte
Adipocytes
Humans
Physical and Theoretical Chemistry
Molecular Biology
lcsh:QH301-705.5
Spectroscopy
Cell growth
Organic Chemistry
Mesenchymal stem cell
Mesenchymal Stem Cells
General Medicine
In vitro
Computer Science Applications
Cell biology
Culture Media
Chemically defined medium
030104 developmental biology
chemistry
lcsh:Biology (General)
lcsh:QD1-999
Immunology
sense organs
Homing (hematopoietic)
Subjects
Details
- Language :
- English
- ISSN :
- 14220067
- Volume :
- 18
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....bc71c3bb47431ffd581135824a94b18f