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Isotopic labelling reveals the efficient adaptation of wheat root TCA cycle flux modes to match carbon demand under ammonium nutrition
- Source :
- Scientific Reports, Scientific Reports, Nature Publishing Group, 2019, 9 (1), pp.8925. ⟨10.1038/s41598-019-45393-8⟩, Addi. Archivo Digital para la Docencia y la Investigación, instname, Scientific Reports 1 (9), . (2019), Scientific Reports, Vol 9, Iss 1, Pp 1-14 (2019)
- Publication Year :
- 2019
- Publisher :
- Nature Publishing Group UK, 2019.
-
Abstract
- Proper carbon (C) supply is essential for nitrogen (N) assimilation especially when plants are grown under ammonium (NH4+) nutrition. However, how C and N metabolic fluxes adapt to achieve so remains uncertain. In this work, roots of wheat (Triticum aestivum L.) plants grown under exclusive NH4+ or nitrate (NO3−) supply were incubated with isotope-labelled substrates (15NH4+, 15NO3−, or [13C]Pyruvate) to follow the incorporation of 15N or 13C into amino acids and organic acids. Roots of plants adapted to ammonium nutrition presented higher capacity to incorporate both 15NH4+ and 15NO3− into amino acids, thanks to the previous induction of the NH4+ assimilative machinery. The 15N label was firstly incorporated into [15N]Gln vía glutamine synthetase; ultimately leading to [15N]Asn accumulation as an optimal NH4+ storage. The provision of [13C]Pyruvate led to [13C]Citrate and [13C]Malate accumulation and to rapid [13C]2-OG consumption for amino acid synthesis and highlighted the importance of the anaplerotic routes associated to tricarboxylic acid (TCA) cycle. Taken together, our results indicate that root adaptation to ammonium nutrition allowed efficient assimilation of N thanks to the promotion of TCA cycle open flux modes in order to sustain C skeleton availability for effective NH4+ detoxification into amino acids.
- Subjects :
- 0301 basic medicine
glutamate synthétase
[SDV]Life Sciences [q-bio]
lcsh:Medicine
isotope du carbone
illuminated leaves
Plant Roots
nitrogen use efficiency
chemistry.chemical_compound
détoxication enzymatique
0302 clinical medicine
Ammonium Compounds
lcsh:Science
Amino acid synthesis
Triticum
2. Zero hunger
chemistry.chemical_classification
mécanisme d'adaptation
Multidisciplinary
food and beverages
Tricarboxylic acid
Nitrogen
Adaptation, Physiological
Amino acid
Biochemistry
Isotope Labeling
analyse de flux
Phosphoenolpyruvate carboxylase
fixation de l'ammonium
asparagine synthetase
inorganic chemicals
Plant physiology
Citric Acid Cycle
chemistry.chemical_element
arabidopsis-thaliana
NAD(H)-dependent glutamate-dehydrogenase
triticum-aestivum L
gaba shunt
Article
03 medical and health sciences
blé
absorption azotée
Glutamine synthetase
tomato plants
Metabolomics
Ammonium
triticum aestivum
phosphoenolpyruvate carboxylase
Nitrates
Mass spectrometry
lcsh:R
Carbon
Citric acid cycle
absorption racinaire
030104 developmental biology
chemistry
nutrition minérale des plantes
amino-acid
lcsh:Q
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....bc40332d18c854f28393fa44f234e044