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Placental transfer of (125)Iodinated humanized immunoglobulin G2Δa in the Sprague Dawley rat
Placental transfer of (125)Iodinated humanized immunoglobulin G2Δa in the Sprague Dawley rat
- Source :
- Reproductive toxicology (Elmsford, N.Y.). 38
- Publication Year :
- 2012
-
Abstract
- Antibody-like biopharmaceuticals cross the placenta by utilizing transport pathways available for transfer of maternal antibodies to the conceptus. To characterize the timing and magnitude of this transfer in the rat, embryo/fetal biodistribution of maternally administered radiolabeled humanized IgG2 was quantified over the course of gestation using gamma counting and whole body autoradiography. The result was humanized IgG2 found in rat embryo/fetal tissues as early as gestation day 11 with a >1000-fold increase in the amount of total IgG2 by day 21. The concentration of IgG2 in rat embryo/fetal tissues generally remained unchanged from gestation day 11 to 17 with a slight increase from day 17 to 21. In addition, fetal-maternal tissue concentration ratios remained stable during organogenesis with a slight increase from gestation day 17 to 21. Based on the empirical amount of antibody present in the embryo/fetus during specific developmental windows, direct antibody binding to biological targets could potentially result in adverse developmental outcomes.
- Subjects :
- medicine.medical_specialty
Placenta
Biology
Toxicology
Antibodies, Monoclonal, Humanized
Immunoglobulin G
Iodine Radioisotopes
Rats, Sprague-Dawley
Fetus
Pregnancy
Internal medicine
medicine
Conceptus
Animals
Tissue Distribution
Maternal-Fetal Exchange
Embryo
medicine.disease
Embryo, Mammalian
Rats
Endocrinology
medicine.anatomical_structure
embryonic structures
biology.protein
Gestation
Autoradiography
Female
Antibody
Subjects
Details
- ISSN :
- 18731708
- Volume :
- 38
- Database :
- OpenAIRE
- Journal :
- Reproductive toxicology (Elmsford, N.Y.)
- Accession number :
- edsair.doi.dedup.....bc131800b68ed0a1a3657b904a7f1f15