Back to Search
Start Over
Dental Enamel Structure Is Altered by Expression of Dominant Negative RhoA in Ameloblasts
- Source :
- Cells Tissues Organs. 194:227-231
- Publication Year :
- 2011
- Publisher :
- S. Karger AG, 2011.
-
Abstract
- Using in vitrotooth germ cultures and analysis by confocal microscopy, ameloblasts treated with sodium fluoride were found to have elevated amounts of filamentous actin. Because this response is reduced by inhibitors of the Rho/ROCK signaling pathway, we generated mice that express dominant negative RhoA (RhoADN) in ameloblasts for in vivo analysis. Expression of the EGFP-RhoADN fusion protein was evaluated by RT-PCR and immunohistochemistry, and teeth were analyzed by scanning electron microscopy. The 3 strains expressed at either low (TgEGFP-RhoADN-8), intermediate (TgEGFP-RhoADN-2), or high (TgEGFP-RhoADN-13) levels, and the molar teeth from the 3 strains had enamel hypoplasia and surface defects. We conclude that RhoADN expressed in ameloblasts interferes with normal enamel development through the pathway that is induced by sodium fluoride.
- Subjects :
- Paper
Molar
Histology
RHOA
Mice, Transgenic
Filamentous actin
law.invention
Mice
chemistry.chemical_compound
stomatognathic system
Confocal microscopy
law
Sodium fluoride
Ameloblasts
medicine
Animals
Transgenes
Dental Enamel
Protein Kinase Inhibitors
Genes, Dominant
Enamel paint
biology
Tooth Germ
Enamel hypoplasia
medicine.disease
Molecular biology
chemistry
Biochemistry
visual_art
visual_art.visual_art_medium
biology.protein
Anatomy
rhoA GTP-Binding Protein
Ameloblast
Subjects
Details
- ISSN :
- 14226421 and 14226405
- Volume :
- 194
- Database :
- OpenAIRE
- Journal :
- Cells Tissues Organs
- Accession number :
- edsair.doi.dedup.....bc0018ecec630add4d3317e431cc14ff