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N-Glycosylation of an IgG antibody secreted by Nicotiana tabacum BY-2 cells can be modulated through co-expression of human β-1,4-galactosyltransferase
- Source :
- Transgenic Research. 26:375-384
- Publication Year :
- 2017
- Publisher :
- Springer Science and Business Media LLC, 2017.
-
Abstract
- Nicotiana tabacum BY-2 suspension cells have several advantages that make them suitable for the production of full-size monoclonal antibodies which can be purified directly from the culture medium. Carbohydrate characterization of an antibody (Lo-BM2) expressed in N. tabacum BY-2 cells showed that the purified Lo-BM2 displays N-glycan homogeneity with a high proportion (>70%) of the complex GnGnXF glycoform. The stable co-expression of a human β-1,4-galactosyltransferase targeted to different Golgi sub-compartments altered Lo-BM2N-glycosylation and resulted in the production of an antibody that exhibited either hybrid structures containing a low abundance of the plant epitopes (α-1,3-fucose and β-1,2-xylose), or a large amount of galactose-extended N-glycan structures. These results demonstrate the suitability of stable N-glycoengineered N. tabacum BY-2 cell lines for the production of human-like antibodies.
- Subjects :
- 0301 basic medicine
Glycosylation
medicine.drug_class
Nicotiana tabacum
Golgi Apparatus
Biology
Monoclonal antibody
Chromatography, Affinity
Epitope
Immunoglobulin G
03 medical and health sciences
chemistry.chemical_compound
N-linked glycosylation
N-Acetyllactosamine Synthase
Tobacco
Genetics
medicine
Humans
fungi
food and beverages
Plants, Genetically Modified
biology.organism_classification
Molecular biology
Recombinant Proteins
030104 developmental biology
Gene Expression Regulation
chemistry
Biochemistry
Cell culture
biology.protein
Animal Science and Zoology
Antibody
Agronomy and Crop Science
Biotechnology
Subjects
Details
- ISSN :
- 15739368 and 09628819
- Volume :
- 26
- Database :
- OpenAIRE
- Journal :
- Transgenic Research
- Accession number :
- edsair.doi.dedup.....bbff1f9e86485d7dcdbf14418b09e584